Evaluation of a Novel Immunochromatographic Assay Using Monoclonal Antibodies against the Matrix Protein of Human Metapneumovirus
| Autor: | Noboru Sakai, Yasuhisa Odagawa, Atsushi Tame, Atsushi Manabe, Daisuke Sato, Hideaki Kikuta, Akiko Okamura, Akihito Ishizaka, Yasushi Akutsu, Takehiro Togashi, Tatsuhiro Tohmoto, Koichi Yasoshima, Nobuhisa Ishiguro, Keisuke Morita, Katsura Ishizu, Motoharu Yonekawa, Tatsuru Yamanaka, Naoko Nagano, Katsuki Azuma, Fujio Kakuya, Akira Tsuchida |
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| Rok vydání: | 2021 |
| Předmět: |
Myeloma protein
medicine.drug_class viruses Monoclonal antibody General Biochemistry Genetics and Molecular Biology Viral Matrix Proteins Human metapneumovirus Nasopharynx Medicine Humans Respiratory Tract Infections Immunoassay Viral matrix protein Paramyxoviridae Infections Respiratory tract infections biology medicine.diagnostic_test business.industry virus diseases Antibodies Monoclonal Infant biology.organism_classification Virology respiratory tract diseases Monoclonal biology.protein Metapneumovirus Antibody business |
| Zdroj: | Clinical laboratory. 67(10) |
| ISSN: | 1433-6510 |
| Popis: | Background The aim of this study was to determine the sensitivity and specificity of a novel immunochromatographic (IC) assay (APD1806) using monoclonal antibodies against the matrix (M) protein of human metapneumovirus (hMPV) for detection of hMPV from nasopharyngeal swab samples based on the results of real-time RT-PCR. Methods Nasopharyngeal swab samples taken from 189 patients aged 0 - 5 years who were suspected of having respiratory tract infections associated with hMPV were used in this study. The samples were tested both by the IC assay and by real-time RT-PCR for detection of hMPV. Results The sensitivity and specificity of the IC assay for detection of hMPV were 88.8% (95/107) and 92.7% (76/82), respectively. Conclusions The IC assay using monoclonal antibodies against the M protein of hMPV is an accurate and fast assay that is suitable as a diagnostic tool for hMPV infection. The optimal timing of the IC assay is 12 hours or more after the onset of fever due to hMPV infection. |
| Databáze: | OpenAIRE |
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