Expression profile of the Kisspeptin/Kiss1r system and angiogenic and immunological mediators in the ovary of cyclic and pregnant cats
| Autor: | Thayná Queiroz Menezes da Silva, Larissa da Silva Santana, Bianca Reis Santos, Erikles Macêdo Barbosa, Elisângela Barboza da Silva, Sophia Saraiva de Souza, Mário Sérgio Lima de Lavor, Luciano Cardoso Santos, Janaína Maria Xavier Corrêa, Juneo Freitas Silva, Jeane Martinha dos Anjos Cordeiro |
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| Rok vydání: | 2022 |
| Předmět: |
endocrine system
medicine.medical_specialty Ovary Biology Luteal phase Endocrinology Kisspeptin Food Animals Corpus Luteum Pregnancy Internal medicine Follicular phase medicine Animals Mammals Estrous cycle Kisspeptins Granulosa Cells CATS medicine.anatomical_structure Cats Female Animal Science and Zoology Folliculogenesis hormones hormone substitutes and hormone antagonists Immunostaining Receptors Kisspeptin-1 |
| Zdroj: | Domestic Animal Endocrinology. 78:106650 |
| ISSN: | 0739-7240 |
| Popis: | The Kisspeptin/Kiss1r system has been studied in mammalian ovaries. However, there are still no studies on the modulation of this system and its relationship with angiogenic and immunological mediators in the ovary of domestic cats, especially during pregnancy. We evaluated the expression of Kisspeptin/Kiss1r and angiogenic and immunological mediators during folliculogenesis, luteogenesis and luteal regression of cyclic and pregnant cats. The ovary exhibited moderate to intense expression for Kiss1, VEGF, Flk-1, INFγ and MIF in oocytes and the follicular wall, while Kiss1r expression was low in granulosa cells. In these cells, there was also a greater expression of Kiss1, INFγ and MIF, mainly in secondary follicles, while tertiary and preovulatory follicles exhibited greater expression of VEGF and Flk-1 in this layer. In luteogenesis, Kiss1 immunostaining was higher in mature corpora lutea (MCL) of pregnant cats compared to vacuolated CL (VCL) and corpus albicans (CA). Pregnancy also increased the luteal gene expression of Kiss1 as well as Kiss1, Kiss1r, Flk-1, and MIF immunostaining in MCL, while reduced the area of VEGF expression in VCL and luteal mRNA expression of Mif when compared to non-pregnant animals. In addition, positive gene correlation between Kiss1r and Mif was observed in the CL. Kiss1, Kiss1r, Vegf and Mif expression were lower in the CA of cats in anestrus. These findings reveal that the expression of Kisspeptin/Kiss1r and angiogenic and immunological mediators, in the ovary of domestic cats, depend on the follicular and luteal stage, and the luteal expression of these mediators is influenced by pregnancy. |
| Databáze: | OpenAIRE |
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