Stratum corneum markers of innate and T helper cell-related immunity and their relation to the disease severity in Croatian patients with atopic dermatitis
| Autor: | Guillem Hurault, S. Ljubojevic Hadzavdic, Reiko J. Tanaka, Branka Marinović, B. Pavicic, K. Zuzul, Sanja Kezic, Y. Sun, Ivone Jakasa, Marija Petković, Anamaria Balić, R. Jurakić Tončić |
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| Přispěvatelé: | Public and occupational health, APH - Societal Participation & Health, APH - Personalized Medicine, AII - Infectious diseases, Coronel Institute of Occupational Health |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_treatment Population biomarkers atopic dermatitis stratum corneum NMF Dermatology Filaggrin Proteins Severity of Illness Index Dermatitis Atopic 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Immune system medicine Humans SCORAD education Skin education.field_of_study Innate immune system Science & Technology medicine.diagnostic_test integumentary system business.industry Dermatology & Venereal Diseases 1103 Clinical Sciences T helper cell Atopic dermatitis T-Lymphocytes Helper-Inducer medicine.disease 030104 developmental biology Infectious Diseases Cytokine medicine.anatomical_structure Immunology Epidermis business Life Sciences & Biomedicine Biomarkers Filaggrin |
| Zdroj: | Journal of the European Academy of Dermatology and Venereology, 35(5), 1186-1196. Wiley-Blackwell |
| ISSN: | 0926-9959 |
| DOI: | 10.1111/jdv.17132 |
| Popis: | Background Atopic dermatitis (AD) presents with the wide spectrum of clinical phenotypes within and between various populations. Recent study showed low frequency of filaggrin loss-of-function (FLG LOF) mutations in Croatian AD patients. At present, there are no data on biomarkers of immune response in Croatian AD patients that might be useful in the selection and monitoring of novel immune therapies. Objectives To investigate levels of cytokines of various signature in the stratum corneum (SC) collected from lesional and non-lesional skin of AD patients and healthy controls, and to evaluate their relationship with the severity of disease and skin barrier function. Methods SC samples were collected from 100 adult patients with moderate to severe AD and 50 healthy controls. The levels of 21 cytokines were measured by multiplex immunoassay. We conducted machine learning analysis to assess whether a small number of cytokine measurements can discriminate between healthy controls and AD patients and can predict AD severity (SCORAD). Results The SC levels of thirteen cytokines representing innate immunity, Th-1, Th-2 and Th-17/22 immune response showed significant differences between healthy and AD skin. Our analysis demonstrated that as few as three cytokines measured in lesional skin can discriminate healthy controls and AD with an accuracy of 99%, and that the predictive models for SCORAD did not achieve a high accuracy. Cytokine levels were highly correlated with the levels of filaggrin degradation products and skin barrier function. Conclusions Stratum corneum analysis revealed aberrant levels of cytokines representing innate immunity, Th-1, Th-2 and Th-17/22 mediated immune response in Croatian AD patients. Increased Th-2 cytokines, and their strong association with natural moisturizing factor (NMF) can explain low NMF levels despite of low frequency of FLG LOF mutations in Croatian population. Predictive models for SCORAD identified cytokines associated with SCORAD but warrants further investigation. |
| Databáze: | OpenAIRE |
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