Elevated integrin α6 expression is involved in the occurrence and development of lung adenocarcinoma, and predicts a poor prognosis: a study based on immunohistochemical analysis and bioinformatics
| Autor: | Chengchu Zhu, Baofu Chen, Jianfei Shen, Jianfeng Xu, Zixuan Chen, Ji-Cheng Li, Dehua Ma |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Cancer Research medicine.medical_specialty Integrin Adenocarcinoma of Lung Kaplan-Meier Estimate Integrin alpha6 03 medical and health sciences 0302 clinical medicine Internal medicine medicine PTEN Humans RNA Messenger Messenger RNA Hematology Lung biology business.industry PTEN Phosphohydrolase Computational Biology General Medicine Middle Aged medicine.disease Prognosis Immunohistochemistry Up-Regulation Survival Rate 030104 developmental biology medicine.anatomical_structure Oncology Tissue Array Analysis 030220 oncology & carcinogenesis biology.protein Cancer research Adenocarcinoma T-stage business Signal Transduction |
| Zdroj: | Journal of cancer research and clinical oncology. 145(7) |
| ISSN: | 1432-1335 |
| Popis: | To study integrin α6 expression in lung adenocarcinoma tissue through comparison with matching adjacent non-cancerous tissues as well as elucidating the correlation between integrin α6 expression with the clinical parameters of lung adenocarcinoma. We also explore the signal pathways associated with integrin α6 up-regulation. The clinical data, cancer tissues, and adjacent non-cancerous tissues of 30 patients diagnosed with lung adenocarcinoma were collected from Taizhou Hospital in Zhejiang Province, China, in 2010. The protein levels of integrin α6 were determined by immunohistochemistry methods. mRNA data of 85 lung adenocarcinoma tissues and 14 normal tissues as well as clinical results were collected from GEO30219. We also collected mRNA data of 533 lung adenocarcinoma tissues and 59 normal tissues as well as the clinical results of 522 patients with lung adenocarcinoma from the Cancer Genome Atlas (TCGA) database. The differences in protein and mRNA levels in cancer tissues and non-cancerous tissues were analyzed, and we subsequently investigated the association between integrin α6 expression and key parameters indicating lung adenocarcinoma progression and overall survival rate. Additionally, the possible pathways involved in the up-regulation of integrin α6 were analyzed by GSEA. The protein levels of integrin α6 in lung adenocarcinoma tissues were significantly higher than those in adjacent tissues (p |
| Databáze: | OpenAIRE |
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