Precocious subcutaneous abdominal stem cell development to adipocytes in normal-weight women with polycystic ovary syndrome
| Autor: | David H. Abbott, Samantha C. Fisch, Gregorio D. Chazenbalk, Daniel A. Dumesic, Elizabeth A. Wright, Karen L. Leung, Julia D. Phan, Tristan Grogan, Ariella Farzan Nikou |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Time Factors Ideal Body Weight Adipose tissue Reproductive health and childbirth Body Mass Index hyperandrogenism chemistry.chemical_compound 0302 clinical medicine Stem Cell Research - Nonembryonic - Human Adipocyte Adipocytes Testosterone Adipogenesis Diabetes Obstetrics and Gynecology Cell Differentiation Abdominal adiposity Polycystic ovary Photon Adult Stem Cells Public Health and Health Services Female Adult medicine.medical_specialty Adolescent medicine.drug_class Clinical Sciences Subcutaneous Fat Abdominal Fat 030209 endocrinology & metabolism adipocyte Paediatrics and Reproductive Medicine 03 medical and health sciences Young Adult Clinical Research Internal medicine medicine Humans Obesity Absorptiometry Obstetrics & Reproductive Medicine Metabolic and endocrine Nutrition business.industry Contraception/Reproduction Hyperandrogenism Glucose Tolerance Test Androgen medicine.disease Stem Cell Research stem cell 030104 developmental biology Endocrinology Reproductive Medicine chemistry polycystic ovary syndrome Case-Control Studies business Body mass index Hormone |
| Zdroj: | Fertility and sterility, vol 110, iss 7 |
| Popis: | Objective To examine whether abnormal subcutaneous (SC) abdominal adipose stem cell (ASC) development to adipocytes in polycystic ovary syndrome (PCOS) correlates with hyperandrogenism. Design Prospective cohort study. Setting Academic medical center. Patient(s) Eight normal-weight women with PCOS and eight normoandrogenic ovulatory (control) women matched for age and body mass index. Intervention(s) Circulating hormone and metabolic measurements, intravenous glucose tolerance testing, total body dual-energy X-ray absorptiometry, and SC abdominal fat biopsy. Main Outcome Measure(s) In vitro ASC commitment to preadipocytes (ZFP423 protein expression, day 0.5), preadipocyte differentiation to adipocytes (PPARγ gene expression, day 3) and adipocyte lipid content (Oil-Red-O fluorescence, day 12) comparisons correlated with clinical outcomes. Result(s) In women with PCOS, SC abdominal ASCs compared with those of control women showed exaggerated commitment to preadipocytes and had greater lipid content in newly formed adipocytes after in vitro maturation. In all women combined, ZFP423 protein expression negatively correlated with fasting plasma glucose levels whereas the lipid content of newly formed adipocytes positively correlated with both PPARγ gene expression and serum free testosterone levels. Conclusion(s) In normal-weight women with PCOS compared with the control group, exaggerated SC abdominal ASC commitment to preadipocytes and enhanced adipocyte lipid content during maturation in vitro negatively and positively correlate with circulating fasting glucose and androgen levels, respectively, as a possible mechanism to maintain glucose–insulin homeostasis when fat accretion is accelerated. |
| Databáze: | OpenAIRE |
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