Organic overloading affects the microbial interactions during anaerobic digestion in sewage sludge reactors
| Autor: | Juan M. Lema, Marta Carballa, Nuria Fernandez-Gonzalez, Guilherme H.R. Braz |
|---|---|
| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Enxeñaría Química |
| Rok vydání: | 2019 |
| Předmět: |
Glycerol
Environmental Engineering Methanogenesis Firmicutes Health Toxicology and Mutagenesis 0208 environmental biotechnology Porphyromonadaceae 02 engineering and technology 010501 environmental sciences Veillonellaceae 01 natural sciences Bioreactors Environmental Chemistry Food science Microbiome Anaerobiosis Anaerobic microbiome 0105 earth and related environmental sciences biology Bacteria Sewage Chemistry Microbiota Public Health Environmental and Occupational Health General Medicine General Chemistry biology.organism_classification Fatty Acids Volatile Methanosaeta Pollution Methanogen 020801 environmental engineering Sludge digestion Anaerobic digestion Fermentation Microbial Interactions Anaerobic exercise Methane Sludge |
| Zdroj: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname |
| Popis: | This is the accepted manuscript of the following article: Braz, G., Fernandez-Gonzalez, N., Lema, J., & Carballa, M. (2019). Organic overloading affects the microbial interactions during anaerobic digestion in sewage sludge reactors. Chemosphere, 222, 323-332. doi: 10.1016/j.chemosphere.2019.01.124 There is still a lack of information about microbial interactions of anaerobic digestion microbiome during process disturbance which limits our ability to predict the mechanisms that drive community dynamics on these events. This paper aims to determine how an organic overloading affects these interactions and to characterize in detail the microbiome structure and diversity in sewage sludge anaerobic reactors during an acidosis event. Two identical sewage sludge anaerobic reactors were subjected to an organic loading shock by adding glycerol waste. As consequence, volatile fatty acids accumulated after only 24 h (up to 2.5 g/L) while Bacteroidales and Methanomicrobiales became displaced by Firmicutes and Methanosaeta sp, showing that reactor acidosis can occur without an immediate decline of this methanogen. Network analysis revealed 9 clusters of co-occurring microorganisms with different behaviors during overloading. At first, Veillonellaceae family, the main glycerol degrading, associated with Candidatus Cloacimonetes, volatile fatty acids fermenters, increased their relative abundance in detriment of the syntrophic bacteria; although as conditions become more acidic, these groups were displaced by other fermenters like Porphyromonadaceae and Chitinophagaceae. Eventually, the methanogenesis failed 72 h after organic overloading, when pH reached values lower than 6. Overall, our results showed a succession of functionally redundant microorganisms, most likely because of niche specialization during organic overloading. The detailed temporal analysis elucidated the processes governing the dynamics anaerobic digestion microbiome, a knowledge required to develop anaerobic digestion management strategies based on its microbiome during process disturbances This research was supported by the Spanish Government (AEI) through DCTI (SmartGreenGas project, 2014-CE224). The authors belong to the Galician Competitive Research Group GRC (ED431C 2017/29) and the Strategic Partnership for Research in Environmental Technologies of the Universidade de Santiago de Compostela (CRETUS, AGRUP2015/02). All these programs are co-founded by FEDER (UE). GHRB PhD fellowship is supported by CAPES (BEX-2160/2015-03) Foundation, Ministry of Education of Brazil, Brasília – DF 70040-020, Brazil. Computational resources were kindly provided by the Galician Center of Supercomputation (CESGA) SI |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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