Isolation of Potential Phages against Multidrug-Resistant Bacterial Isolates: Promising Agents in the Rivers of Kathmandu, Nepal
| Autor: | Shreena Shakya, Anjeela Bhetwal, Anjila Maharjan, Narayan Prasad Parajuli, Sumitra Ghimire, Puspa Raj Khanal, Deepa Satyal |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiological Techniques Klebsiella Article Subject Phage therapy medicine.medical_treatment 030106 microbiology lcsh:Medicine Drug resistance Serratia General Biochemistry Genetics and Molecular Biology Microbiology 03 medical and health sciences Enterobacteriaceae Nepal Rivers Drug Resistance Multiple Bacterial medicine Humans Bacteriophages General Immunology and Microbiology biology Host (biology) lcsh:R General Medicine Antimicrobial biology.organism_classification Virology Anti-Bacterial Agents Multiple drug resistance 030104 developmental biology Bacteria Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2017 (2017) |
| ISSN: | 2314-6141 |
| Popis: | Bacteriophages are being the subject of interest for alternative antimicrobial therapy for infectious diseases in recent years. Therapeutic effectiveness regarding phage therapy is a matter of concern since it is the most promising biological treatment of this era. Hence, the present study was aimed to isolate the potential bacteriophages present in river water samples and to analyze their host range among clinical strains of bacteria. Ten different locations of Kathmandu valley were selected for the collection of river water for the detection of probable phages. Bacteriophages were isolated from water samples using the double agar overlay method. Isolated phages were purified by diluting in the SM-buffer and filtering through 0.22 μm filter. Purified lysate was further processed for analyzing its host range by using spot method. Their host range was characterized against 20 bacterial strains, including multidrug-resistant. Total 67 different phages were isolated against 8 different host organisms. Out of them, forty-seven phages were selected for analyzing its host range. Among them, Serratia phages (ΦSER) had the broad host range infecting 17 different bacterial strains including multidrug-resistant harboring ESBL and MBL genotypes. However, Klebsiella phages (ΦKP) had narrow host range in comparison to other phages. Isolated phages had the potential effect against clinical strains of bacteria along with their broader host spectrum. Most importantly, promising effect against MDR pathogens in this study has raised the probable chances of the utility of these phages for biological control of bacterial infection including MBL and ESBL strains. |
| Databáze: | OpenAIRE |
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