Gradient enhanced-fluidity liquid hydrophilic interaction chromatography of ribonucleic acid nucleosides and nucleotides: A 'green' technique
| Autor: | Michael C. Beilke, Martin J. Beres, Susan V. Olesik |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Green chemistry
Analyte Acetonitriles Resolution (mass spectrometry) Analytical chemistry 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Phase (matter) Acetonitrile Chromatography Nucleotides Viscosity 010405 organic chemistry Liquid gas Chemistry Methanol Hydrophilic interaction chromatography 010401 analytical chemistry Organic Chemistry Water Green Chemistry Technology Nucleosides General Medicine 0104 chemical sciences Solvents RNA Hydrophobic and Hydrophilic Interactions Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 1436:84-90 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2016.01.060 |
| Popis: | A "green" hydrophilic interaction liquid chromatography (HILIC) technique for separating the components of mixtures with a broad range of polarities is illustrated using enhanced-fluidity liquid mobile phases. Enhanced-fluidity liquid chromatography (EFLC) involves the addition of liquid CO2 to conventional liquid mobile phases. Decreased mobile phase viscosity and increased analyte diffusivity results when a liquefied gas is dissolved in common liquid mobile phases. The impact of CO2 addition to a methanol:water (MeOH:H2O) mobile phase was studied to optimize HILIC gradient conditions. For the first time a fast separation of 16 ribonucleic acid (RNA) nucleosides/nucleotides was achieved (16min) with greater than 1.3 resolution for all analyte pairs. By using a gradient, the analysis time was reduced by over 100% compared to similar separations conducted under isocratic conditions. The optimal separation using MeOH:H2O:CO2 mobile phases was compared to MeOH:H2O and acetonitrile:water (ACN:H2O) mobile phases. Based on chromatographic performance parameters (efficiency, resolution and speed of analysis) and an assessment of the environmental impact of the mobile phase mixtures, MeOH:H2O:CO2 mixtures are preferred over ACN:H2O or MeOH:H2O mobile phases for the separation of mixtures of RNA nucleosides and nucleotides. |
| Databáze: | OpenAIRE |
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