A validation study of the 1,2-indandione reagent for operational use in the UK: Part 2 — Optimization of processing conditions
| Autor: | Vaughn G. Sears, Rory Downham, Rachel-May Dyer, Niko Nicolasora, Aoife Luscombe, Laura Hussey |
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| Rok vydání: | 2018 |
| Předmět: |
Validation study
Hot Temperature Materials science Eccrine sweat 01 natural sciences Fluorescence Specimen Handling Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Humans Relative humidity 030216 legal & forensic medicine Amino Acids Dermatoglyphics Sweat Process engineering Alternative methods business.industry 010401 analytical chemistry Humidity Forensic Medicine 0104 chemical sciences Reagent Indans Indicators and Reagents business Law |
| Zdroj: | Forensic Science International. 288:266-277 |
| ISSN: | 0379-0738 |
| Popis: | This paper contains details of work carried out to identify the most effective processing conditions for the optimized 1,2-indandione/zn formulation developed for use under UK conditions. Using direct measurements of fluorescence taken from test spots of amino acids and eccrine sweat during oven processing, complemented with experiments on real fingermarks, it was established that processing temperatures above 120°C in the oven were detrimental to the fluorescence of the developed mark. Alternative methods of development to oven processing were found to be effective, but less controllable. High levels of humidification were also found to be detrimental to the fluorescence of 1,2-indandione developed marks, and oven processing at 100°C and 0% relative humidity is therefore recommended for further studies. It has also been shown that 1,2-indandione can develop fingermarks at temperatures as low as 20°C, making it a candidate for use at crime scenes. |
| Databáze: | OpenAIRE |
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