Functional Restoration of Erectile Function Using End-to-side Autonomic-to-somatic Neurorrhaphy in Rats
| Autor: | Chuanjiang Dong, Ziqiang Dong, Qiaoli Wen, Qing Peng, Zonglan Xie, Fei Xiong, Lusheng Zhang, Zheqi Fan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
Functional impairment Wet weight Urologic Surgical Procedures Male Adult male Somatic cell Urology 030232 urology & nephrology Autonomic Nervous System Neurosurgical Procedures Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Erectile Dysfunction Medicine Somatic nerve Animals Muscle Skeletal business.industry Anastomosis Surgical Anatomy Recovery of Function Erectile function Retrograde tracing Nerve Regeneration Rats 030220 oncology & carcinogenesis Anesthesia Feasibility Studies business End to side anastomosis |
| Zdroj: | Urology. 95 |
| ISSN: | 1527-9995 |
| Popis: | To investigate the feasibility of erectile function rehabilitation using end-to-side autonomic-to-somatic neurorrhaphy in rats.Thirty-six adult male Sprague-Dawley rats were divided into 3 groups (n = 12 per group): in the end-to-side coaptation group, the left L6 and S1 spinal nerves were transected, and the distal stump of L6 ventral root was sutured to L4 ventral root through end-to-side neurorrhaphy; in the no-coaptation group, the rats did not undergo coaptation; and in the control group, the left L6 and S1 spinal nerves were transected, but L6 ventral root was preserved. After 4 months, retrograde tracing, histomorphological technique, mating test, and evaluation of functional properties of the regenerated nerve were performed.Mating test showed a significantly higher intromission behavior rate in the end-to-side coaptation group (41.7%) and control group (58.3%) than in the no-coaptation group (0%) (P .001). Intracavernous pressure in end-to-side coaptation group was 31.6 ± 12.0 mmHg, significantly higher than in the no-coaptation group (3.1 ± 1.4 mmHg), but lower than in the control group (67.9 ± 18.0 mmHg) (P .0001). Retrograde tracing indicated the establishment of the new neural pathway. Axon counting and ultrastructure observation confirmed axonal regeneration in the end-to-side coaptation group. The bilateral tibialis anterior muscles wet weight in the end-to-side coaptation group were 0.6686 ± 0.0427 g and 0.6707 ± 0.0515 g (P = .93). The wet weight and morphology of the tibialis anterior muscles revealed no detrimental effect on the donor nerve.Nerve regeneration can be achieved using end-to-side autonomic-to-somatic neurorrhaphy, and erectile function can be restored without the functional impairment of the donor somatic nerve. |
| Databáze: | OpenAIRE |
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