In Vitro Control of Uropathogenic Microorganisms with the Ethanolic Extract from the Leaves of Cochlospermum regium (Schrank) Pilger
Autor: | Danny Ellen Meireles Leme, Jonas da Silva Mota, Allan Belarmino Rodrigues, Melyssa Negri, Terezinha Inez Estivalet Svidzinski, Adriana Araújo de Almeida-Apolonio, Kelly Mari Pires de Oliveira, Claudia Andrea Lima Cardoso, Fabiana Gomes da Silva Dantas |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Traditional medicine biology Article Subject Chemistry Microorganism Vanillin 030106 microbiology Broth microdilution Biofilm Cochlospermum regium lcsh:Other systems of medicine Antimicrobial biology.organism_classification lcsh:RZ201-999 01 natural sciences 0104 chemical sciences Ames test 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound Complementary and alternative medicine Proanthocyanidin Research Article |
Zdroj: | Evidence-based Complementary and Alternative Medicine : eCAM Evidence-Based Complementary and Alternative Medicine, Vol 2017 (2017) |
ISSN: | 1741-4288 1741-427X |
Popis: | The roots of Cochlospermum regium, popularly known as “algodãozinho-do-cerrado,” are used for the treatment of genitourinary infections. However, the removal of their subterranean structures results in the death of the plant, and the use of the leaves becomes a viable alternative. Therefore, the antimicrobial activity of Cochlospermum regium leaf’s ethanolic extract and its action on the biofilm formation of microorganisms associated with urinary infection were evaluated. The total phenolic compounds, flavoids, and tannins were quantified using the reagents Folin-Ciocalteu, aluminum chloride, and vanillin, respectively. The antimicrobial activity was evaluated by the broth microdilution method and the effect of the extract in the biofilm treatment was measured by the drop plate method. Cytotoxicity was evaluated by the method based on the reduction of MTS and the mutagenicity by the Ames test. The ethanolic extract of C. regium leaves presented 87.4 mg/EQ of flavonoids, 167.2 mg/EAG of total phenolic compounds, and 21.7 mg/ECA of condensed tannins. It presented reduction of the biofilm formation for E. coli and C. tropicalis and antimicrobial action of 1 mg/mL and 0.5 mg/mL, respectively. The extract showed no cytotoxicity and mutagenicity at the concentrations tested. This study demonstrated that C. regium leaves are a viable option for the treatment of genitourinary infections and for the species preservation. |
Databáze: | OpenAIRE |
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