First genetic characterisation of multidrug-resistant Mycobacterium tuberculosis isolates from Algeria

Autor: Emmanuelle Cambau, Djamel Yala, CNR-MyRMA, Mohammed Sebaihia, Feriel Bouziane, Laurent Raskine, Rachida Allem, Sylvain Kumanski, Faiza Mougari, Wladimir Sougakoff
Přispěvatelé: Université Hassiba Ben Bouali de Chlef (UHBC), Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur d'Algérie, Réseau International des Instituts Pasteur (RIIP), CCSD, Accord Elsevier, Centre d'Immunologie et de Maladies Infectieuses (CIMI)
Rok vydání: 2019
Předmět:
Adult
0301 basic medicine
Microbiology (medical)
Lineage (genetic)
Genotype
030106 microbiology
Immunology
Antitubercular Agents
Microbial Sensitivity Tests
Microbiology
Mycobacterium tuberculosis
Young Adult
03 medical and health sciences
0302 clinical medicine
Bacterial Proteins
Next generation sequencing (NGS)-amplicon
Drug Resistance
Multiple
Bacterial
Tuberculosis
Multidrug-Resistant
Humans
Immunology and Allergy
Multidrug-resistant
Multidrug-Resistant Mycobacterium tuberculosis
030212 general & internal medicine
Tuberculosis
Pulmonary
ComputingMilieux_MISCELLANEOUS
Aged
Aged
80 and over
Genetics
biology
INHA
High-Throughput Nucleotide Sequencing
Middle Aged
Amplicon
bacterial infections and mycoses
biology.organism_classification
rpoB
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
3. Good health
Multiple drug resistance
Algeria
Mutation
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Zdroj: Journal of Global Antimicrobial Resistance
Journal of Global Antimicrobial Resistance, 2019, 19, pp.301-307. ⟨10.1016/j.jgar.2019.05.010⟩
Journal of Global Antimicrobial Resistance, Elsevier, 2019, 19, pp.301-307. ⟨10.1016/j.jgar.2019.05.010⟩
ISSN: 2213-7165
DOI: 10.1016/j.jgar.2019.05.010
Popis: Objectives To characterise the genotypes of multidrug-resistant (MDR) Mycobacterium tuberculosis (MTB) isolated in Algeria, where there is a low MDR-MTB incidence rate. Methods Ten MDR isolates and one resistant to isoniazid were investigated by PCR-Sanger sequencing for 10 loci involved in resistance. Amplicon-based next generation sequencing (NGS) of 15 loci was additionally performed on isolates harbouring novel mutations. Results Sanger and amplicon-NGS provided the same results as with GenoType kits. Mutations known to be associated with resistance were described for most isolates: rpoB S531L in seven of 10 rifampicin-R MTB isolates, katG S315T in nine of 11 isoniazid-R, and promoter inhA c-15t in three of 11, embB M306V or M306I in two of two ethambutol-R, rpsL K43R in four of eight or rrs a514c associated with gidB L16R in streptomycin-R, gyrA A90V in the ofloxacin-R pre-XDR isolate. New and rare mutations were also described in rpoB (deletion 512-513-514), katG (S315 R , M126I/ R496L), gidB (V124G, E92A, V139A, G37V), and gyrA (P8A). Mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) profiles were similar for three isolates (lineage Cameroon), indicating a possible clonal diffusion in epidemiologically unrelated patients. Conclusions Resistant MTB isolates in Algeria harbour resistance genotypes similar to other countries, but some rare patterns may result from selection and transmission processes inherent to the country.
Databáze: OpenAIRE
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