Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications
Autor: | Hiep L.X. Vu, Jayeshbhai Chaudhari |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
DNA Complementary Swine animal diseases viruses 030106 microbiology lcsh:QR1-502 Porcine Reproductive and Respiratory Syndrome Mutagenesis (molecular biology technique) Review Genome Viral Biology Virus Replication Genome lcsh:Microbiology Virus 03 medical and health sciences Virology Complementary DNA Animals Porcine respiratory and reproductive syndrome virus Gene Host Microbial Interactions swine viruses virus diseases RNA virus biology.organism_classification Porcine reproductive and respiratory syndrome virus Reverse Genetics Reverse genetics 030104 developmental biology Infectious Diseases PRRSV Mutagenesis Site-Directed |
Zdroj: | Viruses Viruses, Vol 12, Iss 1245, p 1245 (2020) |
ISSN: | 1999-4915 |
Popis: | Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive sense, single-stranded RNA virus that is known to infect only pigs. The virus emerged in the late 1980s and became endemic in most swine producing countries, causing substantial economic losses to the swine industry. The first reverse genetics system for PRRSV was reported in 1998. Since then, several infectious cDNA clones for PRRSV have been constructed. The availability of these infectious cDNA clones has facilitated the genetic modifications of the viral genome at precise locations. Common approaches to manipulate the viral genome include site-directed mutagenesis, deletion of viral genes or gene fragments, insertion of foreign genes, and swapping genes between PRRSV strains or between PRRSV and other members of the Arteriviridae family. In this review, we describe the approaches to construct an infectious cDNA for PRRSV and the ten major applications of these infectious clones to study virus biology and virus–host interaction, and to design a new generation of vaccines with improved levels of safety and efficacy. |
Databáze: | OpenAIRE |
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