A highly contiguous genome assembly of the bat hawkmoth Hyles vespertilio (Lepidoptera: Sphingidae)
| Autor: | Franziska Patzold, David Jebb, Heiko Vogel, Anna K. Hundsdoerfer, Gene Myers, Sylke Winkler, Martin Pippel, Michael Hiller |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Sequence assembly Health Informatics hawkmoth–silk moth comparison Genome browser Moths Data Note 010603 evolutionary biology 01 natural sciences Genome 03 medical and health sciences Chiroptera Animals 030304 developmental biology 0303 health sciences biology Computational Biology Molecular Sequence Annotation Genomics Gene Annotation biology.organism_classification Hyles vespertilio Computer Science Applications gene annotation Vespertilio PacBio long reads Evolutionary biology Bombycoidea genome assembly Reference genome |
| Zdroj: | GigaScience |
| Popis: | Background Adapted to different ecological niches, moth species belonging to the Hyles genus exhibit a spectacular diversity of larval color patterns. These species diverged ∼7.5 million years ago, making this rather young genus an interesting system to study a wide range of questions including the process of speciation, ecological adaptation, and adaptive radiation. Results Here we present a high-quality genome assembly of the bat hawkmoth Hyles vespertilio, the first reference genome of a member of the Hyles genus. We generated 51× Pacific Biosciences long reads with an average read length of 8.9 kb. Pacific Biosciences reads longer than 4 kb were assembled into contigs, resulting in a 651.4-Mb assembly consisting of 530 contigs with an N50 value of 7.5 Mb. The circular mitochondrial contig has a length of 15,303 bp. The H. vespertilio genome is very repeat-rich and exhibits a higher repeat content (50.3%) than other Bombycoidea species such as Bombyx mori (45.7%) and Manduca sexta (27.5%). We developed a comprehensive gene annotation workflow to obtain consensus gene models from different evidence including gene projections, protein homology, transcriptome data, and ab initio predictions. The resulting gene annotation is highly complete with 94.5% of BUSCO genes being completely present, which is higher than the BUSCO completeness of the B. mori (92.2%) and M. sexta (90%) annotations. Conclusions Our gene annotation strategy has general applicability to other genomes, and the H. vespertilio genome provides a valuable molecular resource to study a range of questions in this genus, including phylogeny, incomplete lineage sorting, speciation, and hybridization. A genome browser displaying the genome, alignments, and annotations is available at https://genome-public.pks.mpg.de/cgi-bin/hgTracks?db=HLhylVes1. |
| Databáze: | OpenAIRE |
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