Identification of lncRNAs associated with the pathogenesis of ankylosing spondylitis
| Autor: | Dan Huang, Ying Zhang, Yan Long, Bingxi Bao, Jian Liu, Yanyan Fang, Lei Wan |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
lcsh:Diseases of the musculoskeletal system mRNA lncRNAs Context (language use) Peripheral blood mononuclear cell Pathogenesis 03 medical and health sciences 0302 clinical medicine Rheumatology Downregulation and upregulation Internal medicine medicine Humans Orthopedics and Sports Medicine Spondylitis Ankylosing KEGG 030304 developmental biology Autoimmune disease 0303 health sciences Ankylosing spondylitis business.industry Gene Expression Profiling medicine.disease Up-Regulation 030220 oncology & carcinogenesis Immunology Leukocytes Mononuclear RNA Long Noncoding lcsh:RC925-935 RNA-seq business Research Article |
| Zdroj: | BMC Musculoskeletal Disorders BMC Musculoskeletal Disorders, Vol 22, Iss 1, Pp 1-9 (2021) |
| ISSN: | 1471-2474 |
| Popis: | Background Ankylosing spondylitis (AS) is a chronic autoimmune disease affecting the sacroiliac joint. To date, few studies have examined the association between long non-coding RNAs (lncRNAs) and AS pathogenesis. As such, we herein sought to characterize patterns of AS-related lncRNA expression and to evaluate the potential role played by these lncRNAs in this complex autoimmune context. Methods We conducted a RNA-seq analysis of peripheral blood mononuclear cell (PBMC) samples isolated from five AS patients and corresponding controls. These data were then leveraged to characterize AS-related lncRNA expression patterns. We further conducted GO and KEGG enrichment analyses of the parental genes encoding these lncRNAs, and we confirmed the validity of our RNA-seq data by assessing the expression of six lncRNAs via qRT-PCR in 15 AS and control patient samples. Pearson correlation analyses were additionally employed to examine the associations between the expression levels of these six lncRNAs and patient clinical index values. Results We detected 56,575 total lncRNAs in AS and control patient samples during our initial RNA-seq analysis, of which 200 and 70 were found to be up- and down-regulated (FC > 2 or P Conclusion Overall, our findings highlight several lncRNAs that are specifically expressed in PBMCs of AS patients, indicating that they may play key functions in the pathogenesis of this autoimmune disease. Specifically, we determined that NONHSAT118801.2 and NONHSAT183847.1 may influence the occurrence and development of AS. |
| Databáze: | OpenAIRE |
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