Identification of genes induced by rapid intraoperative tissue expansion in mouse skin
| Autor: | Frank Reilly, Jia Luo, Eugene V. Cilento, Julio Hochberg, Yunfeng Zhu, Joshua Barker |
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| Rok vydání: | 2001 |
| Předmět: |
Male
DNA Complementary Time Factors Myotubularin medicine.medical_treatment Molecular Sequence Data Dermatology Biology Polymerase Chain Reaction Surgical Flaps Catheterization Intraoperative Period Mice Skin Physiological Phenomena Complementary DNA Animals Outbred Strains medicine Animals Insulin Amino Acid Sequence Northern blot Gene Skin Differential display Base Sequence Gene Expression Profiling Intron General Medicine Protein Tyrosine Phosphatases Non-Receptor Peptide Fragments Up-Regulation Cell biology Reverse transcription polymerase chain reaction Long Interspersed Nucleotide Elements Gene Expression Regulation Biochemistry Regional Blood Flow DNA Transposable Elements Protein Tyrosine Phosphatases |
| Zdroj: | Archives of Dermatological Research. 293:560-568 |
| ISSN: | 1432-069X 0340-3696 |
| DOI: | 10.1007/s00403-001-0277-8 |
| Popis: | A method of rapid skin stretching, i.e. hemispherical load cycling with an inflated subcutaneous silicone balloon (Rapid Intraoperative Tissue Expansion or RITE), permits the surgeon to rapidly elongate skin and create a flap of greater length for reconstructive plastic surgery. We have previously developed an experimental mouse model to evaluate RITE, and have shown that rapid stretching prevents ischemia and significantly reduces necrosis. Although the advantages of RITE have been demonstrated both clinically and experimentally, the cellular and molecular mechanisms underlying these benefits were unknown. In the study reported here, we used differential display reverse transcription polymerase chain reaction to identify genes that are specifically induced by RITE. Among four differential gene fragments, the expression of one was confirmed by Northern blot hybridization. The cDNA fragment was extended and the resultant sequence analyzed to reveal induction of truncated long interspersed nucleotide element 1 (LINE-1 or L1). Truncated L1 elements are located inside introns of many genes and among these genes myotubularin and insulin I are known to regulate cell growth. Northern hybridization using specific cDNA probes for myotubularin and insulin I demonstrated that it also was induced by RITE. This is the first reported study to show that L1, myotubularin and insulin I are responsive to rapid hemispherical and not rapid linear stretch. |
| Databáze: | OpenAIRE |
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