Knockdown Epithelial Membrane Protein 1 Suppresses Human Degenerative Intervertebral Disc–Derived Nucleus Pulposus Cell Proliferation
| Autor: | Chuan-Sen Zhang, Zhi-Yong Li, Shao-Hu Xiong, Ming Hu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Gene knockdown
Pathology medicine.medical_specialty epithelial membrane protein 1 intervertebral disc degeneration Cell growth Biomedical Engineering Physical Therapy Sports Therapy and Rehabilitation Intervertebral disc Original Articles Biology Cycloheximide Cell morphology cell survival Epithelial membrane protein-1 Chondrocyte Cell biology nucleus pulposus cells chemistry.chemical_compound cell proliferation medicine.anatomical_structure chemistry Apoptosis medicine Immunology and Allergy |
| Zdroj: | Cartilage |
| ISSN: | 1947-6043 1947-6035 |
| Popis: | Objective: Our previous work showed that epithelial membrane protein 1 (EMP1) is highly expressed in nucleus pulposus of the human degenerative intervertebral disc. The present study was designed to investigate the role of EMP1 in nucleus pulposus cells in intervertebral disc degeneration (IDD). Design: Human nucleus pulposus cells derived from degenerative intervertebral discs were cultured. EMP1 expression was knocked down by lentivirus-mediated specific interfering RNA. Cell morphology was observed, and cell proliferation, apoptosis, and cycle were evaluated. Results: Knockdown of EMP1 inhibited cell proliferation, caused cells to shrink, and accelerated the apoptosis induced by serum deprivation or addition of cycloheximide but did not evoke apoptosis in normal culture conditions. Conclusions: These findings suggest that EMP1 promoted chondrocyte proliferation, survival, and morphological change of cells during IDD, implying that EMP1 may be a target for biological therapy for IDD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|