The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: Molecular cloning, nucleotide sequence, and expression
| Autor: | Bernhard J. Eikmanns, Martin U. Griot, Anthony J. Sinskey, Max T. Follettie |
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| Rok vydání: | 1989 |
| Předmět: |
DNA
Bacterial Genotype genetic structures Carboxy-Lyases Molecular Sequence Data Restriction Mapping Mutant Corynebacterium Molecular cloning Biology behavioral disciplines and activities Corynebacterium glutamicum Transformation Genetic Genetics Amino Acid Sequence Cloning Molecular Molecular Biology Gene Base Sequence Nucleic acid sequence Molecular biology Phosphoenolpyruvate Carboxylase Pyruvate carboxylase Blotting Southern Subcloning Biochemistry Genes Bacterial Cosmid bacteria psychological phenomena and processes Plasmids |
| Zdroj: | Molecular and General Genetics MGG. 218:330-339 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00331286 |
| Popis: | The ppc gene of Corynebacterium glutamicum encoding phosphoenolpyruvate (PEP) carboxylase was isolated by complementation of a ppc mutant of Escherichia coli using a cosmid gene bank of chromosomal C. glutamicum DNA. By subsequent subcloning into the plasmid pUC8 and deletion analysis, the ppc gene could be located on a 3.3 kb SalI fragment. This fragment was able to complement the E. coli ppc mutant and conferred PEP carboxylase activity to the mutant. The complete nucleotide sequence of the ppc gene including 5' and 3' flanking regions has been determined and the primary structure of PEP carboxylase was deduced. The sequence predicts a 919 residue protein product (molecular weight of 103 154) which shows 34% similarity with the respective E. coli enzyme. |
| Databáze: | OpenAIRE |
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