In vivo multi-modal imaging of experimental autoimmune uveoretinitis in transgenic reporter mice reveals the dynamic nature of inflammatory changes during disease progression

Autor: Ian P. Wicks, Paul G. McMenamin, Gabrielle L. Goldberg, John V. Forrester, Xiangting Chen, Claude C.A. Bernard, Jelena Kezic
Rok vydání: 2015
Předmět:
Clinical imaging
Pathology
Time Factors
Neutrophils
Freund's Adjuvant
Multimodal Imaging
Dendritic cells
Experimental autoimmune uveoretinitis
Mice
chemistry.chemical_compound
Microglia
General Neuroscience
Retinal inflammation
3. Good health
medicine.anatomical_structure
Neurology
Disease Progression
Optic nerve
Receptors
Chemokine
Genetically modified mouse
medicine.medical_specialty
Transgene
Immunology
CX3C Chemokine Receptor 1
Mice
Transgenic
Biology
Autoimmune Diseases
Uveitis
Cellular and Molecular Neuroscience
Immune system
In vivo
medicine
Animals
Eye Proteins
Reporter mice
Retina
Macrophages
Research
Retinitis
Retinal Vessels
Retinal
Peptide Fragments
eye diseases
CD11c Antigen
Mice
Inbred C57BL
Retinol-Binding Proteins
Disease Models
Animal
Luminescent Proteins
chemistry
Muramidase
sense organs
Zdroj: Journal of Neuroinflammation
ISSN: 1742-2094
DOI: 10.1186/s12974-015-0235-6
Popis: Experimental autoimmune uveoretinitis (EAU) is a widely used experimental animal model of human endogenous posterior uveoretinitis. In the present study, we performed in vivo imaging of the retina in transgenic reporter mice to investigate dynamic changes in exogenous inflammatory cells and endogenous immune cells during the disease process. Transgenic mice (C57Bl/6 J Cx 3 cr1 GFP/+ , C57Bl/6 N CD11c-eYFP, and C57Bl/6 J LysM-eGFP) were used to visualize the dynamic changes of myeloid-derived cells, putative dendritic cells and neutrophils during EAU. Transgenic mice were monitored with multi-modal fundus imaging camera over five time points following disease induction with the retinal auto-antigen, interphotoreceptor retinoid binding protein (IRBP1–20). Disease severity was quantified with both clinical and histopathological grading. In the normal C57Bl/6 J Cx 3 cr1 GFP/+ mouse Cx3cr1-expressing microglia were evenly distributed in the retina. In C57Bl/6 N CD11c-eYFP mice clusters of CD11c-expressing cells were noted in the retina and in C57Bl/6 J LysM-eGFP mice very low numbers of LysM-expressing neutrophils were observed in the fundus. Following immunization with IRBP1–20, fundus examination revealed accumulations of Cx3cr1-GFP+ myeloid cells, CD11c-eYFP+ cells and LysM-eGFP+ myelomonocytic cells around the optic nerve head and along retinal vessels as early as day 14 post-immunization. CD11c-eYFP+ cells appear to resolve marginally earlier (day 21 post-immunization) than Cx3cr1-GFP+ and LysM-eGFP+ cells. The clinical grading of EAU in transgenic mice correlated closely with histopathological grading. These results illustrate that in vivo fundus imaging of transgenic reporter mice allows direct visualization of various exogenously and endogenously derived leukocyte types during EAU progression. This approach acts as a valuable adjunct to other methods of studying the clinical course of EAU.
Databáze: OpenAIRE
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