Glycan binding profile of a fucolectin-related protein (FRP) encoded by the SP2159 gene of Streptococcus pneumoniae
| Autor: | Albert M. Wu, Su-Chen Li, Yung Liang Chen, Tanuja Singh, Kimberly M. Anderson, Yu Teh Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Glycan Research paper medicine.disease_cause Biochemistry Epitope law.invention lcsh:Biochemistry 03 medical and health sciences Binding profile Glycan recognition epitopes law Streptococcus pneumoniae medicine lcsh:QD415-436 Gene Fucolectin biology Chemistry Polyvalency 030104 developmental biology Fucolectin-related protein Recombinant DNA biology.protein Glycotopes Function (biology) |
| Zdroj: | Biochimie Open Biochimie Open, Vol 6, Iss, Pp 17-23 (2018) |
| ISSN: | 2214-0085 |
| Popis: | The recombinant fucolectin-related protein (FRP) of unknown function, encoded by the SP2159 gene of Streptococcus pneumoniae, was expressed in E. coli. In this study, its glycan-recognition epitopes and their binding potencies were examined by enzyme-linked lectinosorbent and inhibition assays. The results indicate that FRP reacted strongly with human blood group ABH and l-Fucα1→2-active glycotopes and in their polyvalent (super) forms. When expressed by mass relative potency, the binding affinities of FRP to poly-l-Fucα1→glycotopes were about 5.0 × 105 folds higher than that of the mono-l-Fucα1→glycotope form. This unique binding property of FRP can be used as a special tool to differentiate complex forms of l-Fucα1→2 and other forms of glycotopes. Highlights • FRP encoded by the SP2159 gene of Streptococcus pneumoniae shows an unusual blood group A, B, and H binding specificity. • Ley, Leb and H determinants, containing the monomeric forms of Fucα1-2Gal-glycotope were weak ligands for FRP. • Ley, Leb and H determinants, corresponding polyvalent forms exhibit unusual strong interactions with FRP. • Lex [Galβ1-4(LFucα1-3)GlcNAc], Lea [Galβ1-3(LFucα1-4) GlcNAc], A (GalNAcα1-3Gal) and B (Galα1-3Gal) are poor inhibitors. • Lex, Lea, A and B same determinants above enhance the FRP-polyvalent LFucα1-2Gal glycotope interactions. • FRP recognizes only LFucα1→ related ligands, but do not with others, such as Man related glycans. |
| Databáze: | OpenAIRE |
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