Production of bovine insulin-like growth factor 2 (bIGF2) in Escherichia coli
| Autor: | Alan Michael Easton, James K. Gierse, Claire E. Kotts, Barbara Kure Klein, Ramnath Seetharam |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Cytoplasm
Recombinant Fusion Proteins medicine.medical_treatment Molecular Sequence Data Protein Sorting Signals medicine.disease_cause Inclusion bodies law.invention Insulin-Like Growth Factor II law Escherichia coli Genetics medicine Animals Amino Acid Sequence Bovine serum albumin Codon Peptide sequence Base Sequence biology Growth factor General Medicine biology.organism_classification Enterobacteriaceae Molecular biology Insulin-like growth factor 2 biology.protein Recombinant DNA Nucleic Acid Conformation Cattle Cell Division |
| Zdroj: | Gene. 101:291-295 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(91)90426-c |
| Popis: | Bovine insulin-like growth factor 2 (bIGF2) was produced in inclusion bodies in the cytoplasm of Escherichia coli and accumulated at high levels: 20-25% of total Coomassie-stained bacterial protein. The level of accumulation of bIGF2 was affected by the choice of codons in the 5' end of the coding sequence and by a rpoH mutation in the host cells. Purified recombinant bIGF2 had the native N terminus and the same mitogenic activity as that of bIGF2 purified from bovine serum. |
| Databáze: | OpenAIRE |
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