Lycium barbarum polysaccharides attenuate kidney injury in septic rats by regulating Keap1-Nrf2/ARE pathway

Autor: Qi Wu, Lantao Liu, Su-fen Guo, Xue-yong Wang, Tao Zhan, Huizhe Zheng, Zhifang Lang, Xiang-hui Meng, Hongwei Wang, Bin Yan
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
NF-E2-Related Factor 2
medicine.medical_treatment
Blotting
Western
Intraperitoneal injection
Inflammation
Pharmacology
Real-Time Polymerase Chain Reaction
030226 pharmacology & pharmacy
General Biochemistry
Genetics and Molecular Biology
Rats
Sprague-Dawley
Sepsis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Western blot
health services administration
medicine
Animals
General Pharmacology
Toxicology and Pharmaceutics
Kidney
Kelch-Like ECH-Associated Protein 1
medicine.diagnostic_test
biology
business.industry
pathological conditions
signs and symptoms
General Medicine
Acute Kidney Injury
medicine.disease
biology.organism_classification
Ulinastatin
KEAP1
Antioxidant Response Elements
Rats
nervous system diseases
body regions
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
chemistry
Cytokines
population characteristics
Lycium
medicine.symptom
business
Drugs
Chinese Herbal
Signal Transduction
Zdroj: Life Sciences. 242:117240
ISSN: 0024-3205
DOI: 10.1016/j.lfs.2019.117240
Popis: Lycium barbarum polysaccharides (LBP) are derived from Wolfberry and have antioxidant activities. This study aimed to evaluate the efficacy of LBP for kidney injury in a rat model of sepsis. Male rats were divided randomly to control group (Con), LPS group (LPS), ulinastatin group (ULI), low dose LBP group (LBP-1), middle dose LBP group (LBP-2) and high dose LBP group (LBP-3). After intraperitoneal injection of LPS (5 mg/kg) to make sepsis model (LPS group), 10,000 U/kg ulinastatin were given in ULI group, and 200, 400 and 800 mg/kg LBP was given in LBP-1, -2, -3 group, respectively. Serum IL-1β, IL-6, IL-8, TNF-α and NF-κB levels were measured by ELISA. Nrf2, Keap1, NF-κB, HO-1 and NQO1 expression levels were detected by PCR and Western blot analysis. We found that LBP decreased the levels of NF-κB and pro-inflammatory cytokines while attenuated kidney injury. In addition, LBP regulated Keap1-Nrf2/ARE signaling pathway in the kidney. In conclusion, LBP attenuates inflammation injury in the kidney via possible regulation of Keap1-Nrf2/ARE signaling.
Databáze: OpenAIRE
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