Transcriptional regulation of acetyl-CoA carboxylase α isoforms in dairy ewes during conjugated linoleic acid induced milk fat depression
| Autor: | M. Urio, Kevin J. Harvatine, E. Ticiani, Raquel Ferreira, D. E. De Oliveira |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Conjugated linoleic acid Mammary gland Adipose tissue SF1-1100 milk fat depression 03 medical and health sciences chemistry.chemical_compound Mammary Glands Animal Internal medicine Lactation Gene expression medicine Animals Protein Isoforms Linoleic Acids Conjugated Promoter Regions Genetic Fatty acid synthesis Glycoproteins Sheep Lipogenesis 0402 animal and dairy science Acetyl-CoA carboxylase food and beverages Lipid Droplets 04 agricultural and veterinary sciences 040201 dairy & animal science Animal culture Diet lactating ewe Dairying Milk 030104 developmental biology Endocrinology medicine.anatomical_structure Adipose Tissue Gene Expression Regulation chemistry Biochemistry Organ Specificity Dietary Supplements gene expression Female Animal Science and Zoology Glycolipids Acetyl-CoA Carboxylase |
| Zdroj: | Animal, Vol 10, Iss 10, Pp 1677-1683 (2016) |
| ISSN: | 1751-7311 |
| Popis: | Feeding trans-10, cis-12 CLA to lactating ewes reduces milk fat by down-regulating expression of enzymes involved in lipid synthesis in the mammary gland and increases adipose tissue lipogenesis. Acetyl-CoA carboxylase α (ACC-α) is a key regulated enzyme in de novo fatty acid synthesis and is decreased by CLA. In the ovine, the ACC-α gene is expressed from three tissue-specific promoters (PI, PII and PIII). This study evaluated promoter-specific ACC-α expression in mammary and adipose tissue of lactating cross-bred Lacaune/Texel ewes during milk fat depression induced by rumen-unprotected trans-10, cis-12 CLA supplement. In all, 12 ewes arranged in a completely randomized design were fed during early, mid and late lactation one of the following treatments for 14 days: Control (forage+0.9 kg of concentrate on a dry matter basis) and CLA (forage+0.9 kg of concentrate+27 g/day of CLA (29.9% trans-10, cis-12)). Mammary gland and adipose tissue biopsies were taken on day 14 for gene expression analysis by real-time PCR. Milk fat yield and concentration were reduced with CLA supplementation by 27%, 21% and 35% and 28%, 26% and 42% during early, mid and late lactation, respectively. Overall, our results suggest that trans-10, cis-12 CLA down-regulates mammary ACC-α gene expression by decreasing expression from PII and PIII in mammary gland and up-regulates adipose ACC-α gene expression by increasing expression from PI. |
| Databáze: | OpenAIRE |
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