Detection of RNA on northern blots by negative staining with aurintricarboxylic acid
| Autor: | Cornelia Speth, Ilse Oberbäumer |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cyclohexanecarboxylic Acids
RNase P Biophysics Molecular Probe Techniques Biochemistry Cell Line chemistry.chemical_compound Aurintricarboxylic acid Animals Ribonuclease Northern blot Molecular Biology Electrophoresis Agar Gel biology Staining and Labeling Aurintricarboxylic Acid RNA Cell Biology Blotting Northern Molecular biology Blot chemistry biology.protein Nucleic acid DNA |
| Zdroj: | Analytical biochemistry. 185(1) |
| ISSN: | 0003-2697 |
| Popis: | Aurintricarboxylic acid (ATA) is a well-known inhibitor of RNA and DNA modifying enzymes and was suggested as a potent RNase inhibitor for preparation of RNA (Hallick et al., 1977, Nucleic Acids Res. 4, 3055-3064). We show that ATA is a very useful stain for detecting RNA on Northern blots and slot blots although it did not fully protect purified RNA in concentrated solution against RNase A. |
| Databáze: | OpenAIRE |
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