Chronic kidney disease aggravates arteriovenous fistula damage in rats
| Autor: | Christian Heiss, Michael J. Jacobs, Tina Kessler, Maria Kokozidou, Thomas A. Koeppel, Stephan Langer, Jennifer Kranz, Christina Lente, Thilo Krüger, Niklas Paulus |
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| Přispěvatelé: | Biochemie, Surgery, RS: CARIM School for Cardiovascular Diseases |
| Jazyk: | angličtina |
| Předmět: |
Pathology
medicine.medical_specialty Normal diet Fistula Femoral vein Arteriovenous fistula Blood Pressure Femoral artery Constriction Pathologic Rats Sprague-Dawley Arteriovenous Shunt Surgical Internal medicine medicine.artery dialysis access medicine Animals arteriovenous fistula Ultrasonography Neointimal hyperplasia Vascular disease business.industry Adenine Calcinosis vascular access Femoral Vein medicine.disease Rats Femoral Artery Disease Models Animal Nephrology Regional Blood Flow arteriovenous shunt Chronic Disease Cardiology Female Kidney Diseases business chronic kidney disease Kidney disease |
| Zdroj: | Kidney International, 78(12), 1312-1321. Elsevier Science |
| ISSN: | 0085-2538 |
| DOI: | 10.1038/ki.2010.353 |
| Popis: | Neointimal hyperplasia (NIH) and impaired dilatation are important contributors to arteriovenous fistula (AVF) failure. It is unclear whether chronic kidney disease (CKD) itself causes adverse remodeling in arterialized veins. Here we determined if CKD specifically triggers adverse effects on vascular remodeling and assessed whether these changes affect the function of AVFs. For this purpose, we used rats on a normal diet or on an adenine-rich diet to induce CKD and created a fistula between the right femoral artery and vein. Fistula maturation was followed noninvasively by high-resolution ultrasound (US), and groups of rats were killed on 42 and 84 days after surgery for histological and immunohistochemical analyses of the AVFs and contralateral femoral vessels. In vivo US and ex vivo morphometric analyses confirmed a significant increase in NIH in the AVFs of both groups with CKD compared to those receiving a normal diet. Furthermore, we found using histological evaluation of the fistula veins in the rats with CKD that the media shrank and their calcification increased significantly. Afferent artery dilatation was significantly impaired in CKD and the downstream fistula vein had delayed dilation after surgery. These changes were accompanied by significantly increased peak systolic velocity at the site of the anastomosis, implying stenosis. Thus, CKD triggers adverse effects on vascular remodeling in AVFs, all of which contribute to anatomical and/or functional stenosis. Kidney International (2010) 78, 1312-1321; doi:10.1038/ki.2010.353; published online 29 September 2010 |
| Databáze: | OpenAIRE |
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