Synthesis and characterization of biomimetic nanogels for immunorecognition
| Autor: | Muriel Lansalot, Claudia S. O. Silva, M. Ângela Taipa, José M. G. Martinho, Jaqueline Q. Garcia |
|---|---|
| Přispěvatelé: | Laboratoire de Chimie, Catalyse, Polymères et Procédés, R 5265 (C2P2), Centre National de la Recherche Scientifique (CNRS)-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC), CQFM, Centro de Química-Física Molecular, IN - Institute of Nanosciences and Nanotechnology, Centro de Química-Física Molecular |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Hydrochloride
Acrylic Resins 02 engineering and technology 010402 general chemistry Ligands 01 natural sciences chemistry.chemical_compound Colloid and Surface Chemistry Biomimetic Materials Polymer chemistry Methacrylamide Humans Colloids Physical and Theoretical Chemistry Particle Size ComputingMilieux_MISCELLANEOUS Cationic polymerization Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences [CHIM.POLY]Chemical Sciences/Polymers chemistry Covalent bond Immunoglobulin G Precipitation polymerization Methacrylates Nanoparticles Amine gas treating 0210 nano-technology Selectivity Gels Biotechnology Nanogel |
| Zdroj: | Colloids and Surfaces B: Biointerfaces Colloids and Surfaces B: Biointerfaces, Elsevier, 2013, 112, pp.264-271. ⟨10.1016/j.colsurfb.2013.08.003⟩ |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2013.08.003⟩ |
| Popis: | Biomimetic nanoparticles are promising materials for biomedical and biotechnological applications. Cationic poly(N-isopropylacrylamide) (PNIPAM) nanogels containing charged amine groups brought by addition of 2-aminoethylmethacrylate hydrochloride (AEMH) or N-(3-aminopropyl) methacrylamide hydrochloride (APMH) as co-monomers were prepared by surfactant-free precipitation polymerization. The influence of the relative amount and mode of addition of the co-monomer on both the size and the amine group density of the nanogel particles was studied. Two nanogels, one prepared using APMH (1%mol/mol NIPAM, in batch) and another with AEMH (2%mol/mol NIPAM, by shot addition) as co-monomers, were selected for the covalent coupling of a Protein L-mimic ligand to free amine groups on the particles. The ability of the synthesized biomimetic nanoparticles for recognizing and binding human IgG (hIgG) molecules was assessed and the selectivity toward immunoglobulin molecules evaluated. |
| Databáze: | OpenAIRE |
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