Modular model-based design for heterologous bioproduction in bacteria
| Autor: | Guillermo Rodrigo, Javier Carrera, Boris Kirov, Thomas E. Landrain, Alfonso Jaramillo |
|---|---|
| Přispěvatelé: | Programme d'Épigénomique, Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Instituto de Biología Molecular y Celular de Plantas, Universitat Politècnica de València (UPV), Instituto ITACA, Laboratoire de Biochimie de l'Ecole polytechnique (BIOC), Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
MESH: Enzymes
Biomedical Engineering Heterologous Bioengineering Context (language use) Computational biology Protein Engineering 03 medical and health sciences Synthetic biology Combinatorial design Model-based design [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology 030304 developmental biology MESH: Gene Expression Regulation Bacterial 0303 health sciences Bacteria biology 030306 microbiology business.industry Gene Expression Regulation Bacterial Modular design biology.organism_classification Bioproduction Enzymes Biotechnology MESH: Protein Engineering MESH: Bacteria MESH: Biotechnology business |
| Zdroj: | Current Opinion in Biotechnology Current Opinion in Biotechnology, Elsevier, 2009, 20 (3), pp.272-9. ⟨10.1016/j.copbio.2009.06.003⟩ Current Opinion in Biotechnology, 2009, 20 (3), pp.272-9. ⟨10.1016/j.copbio.2009.06.003⟩ |
| ISSN: | 0958-1669 |
| DOI: | 10.1016/j.copbio.2009.06.003 |
| Popis: | International audience; We review the current status of expression of heterologous systems for bioenergy and bioproduction in bacteria using a model-based approach. As an aim for synthetic biology, it requires mathematical models of genetic modules that could be characterized independently of their context. This fastens the design of metabolic circuits using a combinatorial design approach, where given pathways could be optimized for maximal bioproduction, while being nontoxic for the chassis. We show how recent characterization of genetic parts, such as promoters, RBS or sRNAs could be used to fine-tune the expression of individual genes to achieve that goal. We also present lists of enzymes that are used for bioproduction, enlarging such set of biological parts. |
| Databáze: | OpenAIRE |
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