Expression of a Lactococcus lactis Phage Resistance Mechanism by Streptococcus thermophilus

Autor:	Peter A. Vandenbergh, Shirley A. Walker, Ebenezer R. Vedamuthu, Sylvain Moineau, Barbara J. Holler
Rok vydání:	1995
Předmět:	Streptococcus thermophilus Ecology viruses Lactococcus lactis food and beverages Biology Molecular cloning biology.organism_classification Streptococcaceae Applied Microbiology and Biotechnology Microbiology Bacteriophage Plasmid Gene expression Bacteria Research Article Food Science Biotechnology
Zdroj:	Scopus-Elsevier
ISSN:	1098-5336 0099-2240
DOI:	10.1128/aem.61.7.2461-2466.1995
Popis:	The 7.8-kb lactococcal plasmid pSRQ700 encodes the LlaII restriction/modification system which recognizes and cleaves the sequence 3(prm1)-GATC-5(prm1). When the plasmid pSRQ700 is introduced into a phage-sensitive Lactococcus lactis strain, strong phage resistance is conferred by the LlaII system. In this report, we show that pSRQ700 cannot replicate in Streptococcus thermophilus. However, if cloned into the vector pNZ123, the native LlaII system is expressed and strong phage resistance is conferred to various industrial S. thermophilus strains. Resistance against phages isolated from yogurt and mozzarella wheys was observed. To our knowledge, this is the first report of increased phage resistance in S. thermophilus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3981d7ccf4dec0c0efc28634ae813f3 https://doi.org/10.1128/aem.61.7.2461-2466.1995 Zobrazit plný text záznamu