STAT3 but Not ERK2 Is a Crucial Mediator Against Diet-Induced Obesity via VMH Neurons
| Autor: | Gislaine Almeida-Pereira, José Antunes-Rodrigues, Jose Donato, Beatriz C. Borges, Gabriel Henrique Marques Gonçalves, Margaret de Castro, Sabrina Mara Tristão, Lucila Leico Kagohara Elias, Rafaella Eduarda Volpi |
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| Rok vydání: | 2021 |
| Předmět: |
Male
STAT3 Transcription Factor Steroidogenic factor 1 endocrine system medicine.medical_specialty Endocrinology Diabetes and Metabolism Biology Diet High-Fat Steroidogenic Factor 1 Energy homeostasis Mice Mediator Internal medicine Conditional gene knockout Internal Medicine medicine Animals Glucose homeostasis Obesity NEURÔNIOS STAT3 Mitogen-Activated Protein Kinase 1 Sex Characteristics Leptin Mice Inbred C57BL Endocrinology Ventromedial Hypothalamic Nucleus biology.protein Female RNA Splicing Factors Energy Metabolism hormones hormone substitutes and hormone antagonists Intracellular |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/db20-0658 |
| Popis: | Leptin plays an important role in the protection against diet-induced obesity (DIO) by its actions in ventromedial hypothalamic (VMH) neurons. However, little is known about the intracellular mechanisms involved in these effects. To assess the role of the STAT3 and ERK2 signaling in neurons that express the steroidogenic factor 1 (SF1) in the VMH on energy homeostasis, we used cre-lox technology to generate male and female mice with specific disruption of STAT3 or ERK2 in SF1 neurons of the VMH. We demonstrated that the conditional knockout of STAT3 in SF1 neurons of the VMH did not affect body weight, food intake, energy expenditure and glucose homeostasis in animals on regular chow. However, when challenged with high-fat diet (HFD), loss of STAT3 in SF1 neurons caused a significant increase in body weight, food intake and energy efficiency that was more remarkable in females which also showed a decrease in energy expenditure. In contrast, deletion of ERK2 in SF1 neurons of VMH did not have any impact on energy homeostasis in both regular diet and HFD conditions. In conclusion, STAT3 but not ERK2 signaling in SF1 neurons of VMH plays a crucial role to protect against DIO in a sex-specific pattern. |
| Databáze: | OpenAIRE |
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