Intracoronary monocyte expression pattern and HDL subfractions after non-ST elevation myocardial infarction
| Autor: | Antonio Moschetta, Pasquale Caldarola, Lucilla Crudele, Jennifer Härdfeldt, Giusi Graziano, Alice Ossoli, Marilidia Piglionica, Roberta Zerlotin, Maria Arconzo, Michele Vacca, Roberto Salvia, Carlo Sabbà, Laura Calabresi, Stefano Battaglia, David Rutigliano, Marica Cariello |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Apolipoprotein E medicine.medical_specialty 030204 cardiovascular system & hematology Monocytes Coronary artery disease 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Humans Myocardial infarction Non-ST Elevated Myocardial Infarction Liver X receptor Molecular Biology biology business.industry Cholesterol Unstable angina Cholesterol HDL Reverse cholesterol transport Middle Aged medicine.disease Coronary Vessels Lipids Cross-Sectional Studies 030104 developmental biology chemistry ABCA1 Cardiology biology.protein Molecular Medicine Female lipids (amino acids peptides and proteins) business |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1867:166116 |
| ISSN: | 0925-4439 |
| DOI: | 10.1016/j.bbadis.2021.166116 |
| Popis: | Aims Coronary artery disease (CAD) is described as a range of clinical conditions including myocardial infarction (MI) and unstable angina. Lipid and apolipoprotein profiles together with the study of cholesterol deposit and efflux serve to identify novel pre and post infarct scenarios for the treatment of these patients. In (non-ST elevation myocardial infarction) NSTEMI patients, we analysed both systemic and intracoronary serum ability to accept cholesterol as well as cholesterol efflux capacity (CEC) of monocytes in terms of expression of genes involved in the reverse cholesterol transport (RCT). Methods and results While HDL-C quantity was similar between systemic and coronary arterial blood, in 21 NSTEMI patients we observed a significant reduction of the preβ-HDL fraction and the levels of Apolipoproteins AI, AII, B and E in coronary versus systemic serum. These data are complemented with the observed reduction of CEC. On the contrary, compared to systemic arterial monocytes, in coronary microenvironment of NSTEMI patients after myocardial infarction, the monocytes exhibited a higher mRNA expression of nuclear receptor LXRα and its targets ABCA1 and APOE, which drive cholesterol efflux capacity. Conclusion In this cross-sectional study we observe that in the immediate post infarction period, there is a spontaneous bona fide ligand-induced activation of the LXR driven cholesterol efflux capacity of intracoronary monocytes to overcome the reduced serum ability to accept cholesterol and to inhibit the post-infarction pro-inflammatory local microenvironment. |
| Databáze: | OpenAIRE |
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