Expression and Antibody Preparation of GP5a Gene of Porcine Reproductive and Respiratory Syndrome Virus
| Autor: | Liguo Yuan, Chunya Wei, Haitao Qi, Ye Chen, Chao-Yi Zhang, Guihong Zhang, Liangquan Zhang, Zhen Huang, Zhangyong Ning, Long Sun, Wenbao Qi, Jiexiong Xie, Heng Wang, Minze Zhang |
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| Rok vydání: | 2013 |
| Předmět: |
biology
animal diseases Short Communication Respiratory disease Porcine reproductive and respiratory syndrome virus biology.organism_classification medicine.disease Microbiology Virology chemistry.chemical_compound chemistry Transcription (biology) RNA polymerase Gene duplication biology.protein medicine Antibody Respiratory system Gene |
| Zdroj: | Indian journal of microbiology. 53(3) |
| ISSN: | 0046-8991 |
| Popis: | Porcine reproductive and respiratory syndrome (PRRS) is considered one of the most important infectious diseases to affect the swine industry and characterized by reproductive failure in late term gestation in sows and respiratory disease in pigs of all ages. The GP5a gene, encoding RNA-dependent RNA polymerase, is generally regarded as fairly conserved when compared to other viral proteins. It plays an important role in the process of duplication and transcription carried out by Porcine reproductive and respiratory syndrome virus (PRRSV). We firstly expressed and purified the GP5a protein of PRRSV. This provides a good method for the purification of expressed proteins and the preparation of the corresponding antibodies. |
| Databáze: | OpenAIRE |
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