Detection of the mcr-1 gene in Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains isolated from broilers
| Autor: | Virginia Léo de Almeida Pereira, Dayse Lima da Costa Abreu, Ana C.L.Q. Pinto, Nathalie Costa da Cunha, Gisllany Alves Costa, Leandro dos Santos Machado, Elmiro Rosendo do Nascimento, Hugo Peralva Lopes |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
stec shigatoxigenic animal diseases chicken 030106 microbiology detection Biology mcr-1 Microbiology strains 03 medical and health sciences epec Escherichia coli Enteropathogenic Escherichia coli Shigatoxigenic genes Gene EPEC lcsh:Veterinary medicine General Veterinary broilers enteropathogenic biochemical phenomena metabolism and nutrition bacterial infections and mycoses Detection STEC 030104 developmental biology lcsh:SF600-1100 bacteria MCR-1 escherichia coli |
| Zdroj: | Pesquisa Veterinária Brasileira v.40 n.3 2020 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Vol 40, Iss 3, Pp 165-169 |
| Popis: | Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains are among the major pathotypes found in poultry and their products, which are capable of causing human enteric infections. Colistin has been claimed the drug of choice against diseases caused by multidrug-resistant Gram-negative bacteria (MDRGN) in humans. The mcr-1 gene was the first plasmidial gene that has been described to be responsible for colistin resistance and has also been detected in birds and poultry products. Our study aimed to detect the mcr-1 gene in enteropathogenic strains of E. coli in order to evaluate the resistance to colistin in broilers. The material was obtained from 240 cloacal samples and 60 broiler carcasses. The strains were isolated by the conventional bacteriological method and by the virulence genes, which characterize the enteropathogenic strains and resistance, and the samples were detected by polymerase chain reaction (PCR). Of the 213 isolated strains of E. coli, 57 (26.76%) were characterized as atypical EPEC and 35 (16.43%) as STEC. The mcr-1 gene was found in 3.5% (2/57) of the EPEC strains and 5.7% (2/35) of the STEC strains. In this study, it was possible to confirm that the mcr-1 resistance gene is already circulating in the broiler flocks studied and may be associated with the pathogenic strains. |
| Databáze: | OpenAIRE |
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