2,4-Dichlorophenol Enzymatic Removal and Its Kinetic Study Using Horseradish Peroxidase Crosslinked to Nano Spray-Dried Poly(Lactic-Co-Glycolic Acid) Fine Particles
| Autor: | Tivadar Feczkó, Endre Nagy, Laura Amina Dahili |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Immobilized enzyme
020209 energy 02 engineering and technology Wastewater Applied Microbiology and Biotechnology Horseradish peroxidase Armoracia Catalysis Water Purification chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Nano spray dryer Polymer chemistry Enzyme Stability 0202 electrical engineering electronic engineering information engineering Lactic Acid Hydrogen peroxide Glycolic acid Horseradish Peroxidase Enzyme Assays biology Plant Extracts Temperature food and beverages General Medicine Hydrogen-Ion Concentration Enzymes Immobilized PLGA Kinetics chemistry Product inhibition biology.protein Microscopy Electron Scanning Nanoparticles Adsorption Dichlorophenol Polyglycolic Acid Biotechnology Nuclear chemistry Chlorophenols |
| Zdroj: | Journal of microbiology and biotechnology. 27(4) |
| ISSN: | 1738-8872 |
| Popis: | Horseradish peroxidase (HRP) catalyzes the oxidation of aromatic compounds by hydrogen peroxide via insoluble polymer formation, which can be precipitated from the wastewater. For HRP immobilization, poly(lactic-co-glycolic acid) (PLGA) fine carrier supports were produced by using the Nano Spray Dryer B-90. Immobilized HRP was used to remove the persistent 2,4- dichlorophenol from model wastewater. Both extracted (9-16 U/g) and purified HRP (11- 25 U/g) retained their activity to a high extent after crosslinking to the PLGA particles. The immobilized enzyme activity was substantially higher in both the acidic and the alkaline pH regions compared with the free enzyme. Optimally, 98% of the 2,4-dichlorophenol could be eliminated using immobilized HRP due to catalytic removal and partly to adsorption on the carrier supports. Immobilized enzyme kinetics for 2,4-dichlorophenol elimination was studied for the first time, and it could be concluded that competitive product inhibition took place. |
| Databáze: | OpenAIRE |
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