Sustained increase of Ca+2 oscillations after chronic TRPV1 receptor activation with capsaicin in cultured spinal neurons
| Autor: | Jorge Roa, Patricio A. Castro, Fernando J. Sepúlveda, Gretchen Wandersleben, Luis G. Aguayo, Carlos Larrucea |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
TRPV1 TRPV Cation Channels Tetrodotoxin Neurotransmission CREB chemistry.chemical_compound Mice Pregnancy Internal medicine Ca2+/calmodulin-dependent protein kinase medicine Animals Humans Drug Interactions Enzyme Inhibitors Molecular Biology Cells Cultured 6-Cyano-7-nitroquinoxaline-2 3-dione Neurons Analysis of Variance biology Dose-Response Relationship Drug Chemistry General Neuroscience CREB-Binding Protein Ruthenium Red Mice Inbred C57BL Endocrinology Allodynia Spinal Cord Capsaicin Hyperalgesia biology.protein Calcium Female Neurology (clinical) medicine.symptom Capsazepine Excitatory Amino Acid Antagonists Developmental Biology Sodium Channel Blockers |
| Zdroj: | Brain research. 1218 |
| ISSN: | 0006-8993 |
| Popis: | Hyperalgesia and allodynia occur as a consequence of peripheral and central sensitization that follows sustained nociceptive activation. The cellular alterations associated to this state of nociceptive network hyperexcitability represent a form of neuronal plasticity, but they are not well understood because of its complexity in situ. In this study, after treating primary spinal neuron cultures with capsaicin (0.5-1 microM) for 48 h fluorimetric recordings were performed. The activation of TRPV1 receptors with capsaicin (0.5-1.0 microM) increased the frequency of calcium transients (0.03+/-0.002 Hz vs. 0.05+/-0.006 Hz, P |
| Databáze: | OpenAIRE |
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