In vitro multiplication of Eucalyptus hybrid via temporary immersion bioreactor: culture media and cytokinin effects
| Autor: | Evânia Galvão Mendonça, Wesley Pires Flausino Máximo, Paulo Augusto Almeida Santos, Luciano Vilela Paiva, Guilherme Silva Martins |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
plant biotechnology Biology 01 natural sciences chemistry.chemical_compound Axillary bud Bioreactor hyperhydricity in vitro propagation General Environmental Science Hyperhydricity fungi food and beverages 04 agricultural and veterinary sciences In vitro Horticulture chemistry 6-benzilaminopurine Cytokinin Shoot 040103 agronomy & agriculture 0401 agriculture forestry and fisheries General Earth and Planetary Sciences Kinetin Agronomy and Crop Science 010606 plant biology & botany Biotechnology Explant culture |
| Zdroj: | Crop Breeding and Applied Biotechnology v.18 n.2 2018 Crop Breeding and Applied Biotechnology Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB Crop Breeding and Applied Biotechnology, Volume: 18, Issue: 2, Pages: 131-138, Published: APR 2018 |
| Popis: | This study aimed to assess the influence of culture medium and cytokinin type on in vitro multiplication of an Eucalyptus grandis x E. urophylla hybrid clone via temporary immersion bioreactor (TIB®). JADS, modified MS at a (NO3ˉ):(NH +) ratio, and WPM were used as liquid media; and 6-benzilaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ) were the cytokinins used in the study. According to the results, explants under the influence of modified MS presented better results for all traits analyzed in this study. BAP was the most suitable plant growth regulator for axillary bud shoot proliferation. By the leaf histological analysis, most of the shoots grown in the presence of BAP either exhibited normal morphology or only a few hyperhydricity symptoms. All these results were obtained at 19 days after in vitro cultivation, a shorter period when compared with other works in the literature, showing the protocol efficiency in the multiplication of this hybrid clone using TIB®. |
| Databáze: | OpenAIRE |
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