Influence of bacterial lectin on key regulatory links of functional activity of macrophages in mice with Ehrlich carcinoma
| Autor: | Vasyl F. Chekhun, О М Karaman, N L Cheremshenko, V M Shcherbina, N I Fedosova, A V Chumak |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Cancer Research medicine.medical_treatment Nitric Oxide Mice Lectins Carcinoma medicine Animals Cytotoxic T cell STAT1 Carcinoma Ehrlich Tumor Transcription factor Mice Inbred BALB C Arginase biology Chemistry Lectin Immunotherapy Macrophage Activation medicine.disease Molecular biology STAT1 Transcription Factor Gene Expression Regulation Oncology Interferon Regulatory Factors biology.protein STAT6 Transcription Factor IRF5 Bacillus subtilis |
| Zdroj: | Experimental Oncology. |
| ISSN: | 1812-9269 |
| Popis: | BACKGROUND Recent studies have shown the potential of using different approaches for immunotherapy in cancer treatment. Macrophages (Mph) are one of the promising targets for immunotherapy. AIM To investigate changes in the functional activity of Mph in mice with Ehrlich carcinoma by nitric oxide (NO)/arginase (Arg), IRF4/IRF5 and STAT1/STAT6 ratios caused by administration of lectin from B. subtilis IMV-7724. MATERIALS AND METHODS From the 2nd day after Ehrlich carcinoma inoculation into female Balb/c mice, lectin from B. subtilis IMV B-7724 (0.02 mg/mouse) was administered for 10 days. The peritoneal Mph were isolated on days 14, 21, and 28 after tumor transplantation and their functional state (NO production, Arg activity and cytotoxic activity) was examined. The levels of mRNA expression of transcription factors STAT-1, STAT-6, IRF5, IRF4 were evaluated. RESULTS In lectin-treated animals with Ehrlich carcinoma, the functional state of Mph (NO/Arg ratio, index of cytotoxic activity) was maintained at the level of intact mice exceeding the values in untreated animals with Ehrlich carcinoma at late terms of tumor growth (21, 28 days). Analysis of mRNA expression levels of transcription factors in these animals showed a significant increase (p < 0.05) in the ratio of STAT1/STAT6 on the day 21 and IRF5/IRF4 on day 28 of tumor growth compared to that in untreated mice. CONCLUSIONS Administration of lectin from B. subtilis IMV B-7724 to mice with Ehrlich carcinoma led to the prevalence of Mph exhibiting the functional properties of M1 type at late-term tumor growth. The transcription factors of the STAT and IRF signaling pathways are involved in the process of Mph polarization induced by lectin from B. subtilis IMV B-7724. |
| Databáze: | OpenAIRE |
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