Single droplet detection of immune checkpoints on a multiplexed electrohydrodynamic biosensor
| Autor: | Abu Ali Ibn Sina, Aswin Raj Rajkumar, Shuvashis Dey, K. Kamil Reza, Matt Trau, Alain Wuethrich, Karthik Balaji Shanmugasundaram, Christopher B. Howard |
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| Rok vydání: | 2019 |
| Předmět: |
Bioanalysis
medicine.drug_class Microfluidics Programmed Cell Death 1 Receptor 02 engineering and technology Biosensing Techniques Monoclonal antibody 01 natural sciences Biochemistry Armoracia B7-H1 Antigen Analytical Chemistry Immune system Antigens CD Lab-On-A-Chip Devices Electrochemistry medicine Biomarkers Tumor Environmental Chemistry Humans Liquid biopsy Spectroscopy Horseradish Peroxidase Detection limit Immunoassay Chemistry Benzidines 010401 analytical chemistry Electrochemical Techniques Hydrogen Peroxide 021001 nanoscience & nanotechnology Lymphocyte Activation Gene 3 Protein Immune checkpoint 0104 chemical sciences 3. Good health Cell biology Hydrodynamics Colorimetry 0210 nano-technology Biosensor Single-Chain Antibodies |
| Zdroj: | The Analyst. 144(23) |
| ISSN: | 1364-5528 |
| Popis: | Monitoring soluble immune checkpoints in circulating fluids has the potential for minimally-invasive diagnostics and personalised therapy in precision medicine. Yet, the sensitive detection of multiple immune checkpoints from small volumes of liquid biopsy samples is challenging. In this study, we develop a multiplexed immune checkpoint biosensor (MICB) for parallel detection of soluble immune checkpoints PD-1, PD-L1, and LAG-3. MICB integrates a microfluidic sandwich immunoassay using engineered single chain variable fragments and alternating current electrohydrodynamic in situ nanofluidic mixing for promoting biosensor-target interaction and reducing non-specific non-target binding. MICB provides advantages of simultaneous analysis of up to 28 samples in |
| Databáze: | OpenAIRE |
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