The determination of cortisol in fish plasma by radioimmunoassay
| Autor: | H.C. Freeman, G.B. Sangalang, M. McMenemy, R.B. Flemming |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Antiserum
endocrine system medicine.medical_specialty Ammonium sulfate Chromatography biology Hydrocortisone Fishes Radioimmunoassay biology.organism_classification Thin-layer chromatography chemistry.chemical_compound Endocrinology chemistry Internal medicine medicine Gadus Fish Animals Animal Science and Zoology Chromatography Thin Layer hormones hormone substitutes and hormone antagonists |
| Zdroj: | General and comparative endocrinology. 40(4) |
| ISSN: | 0016-6480 |
| Popis: | A radioimmunoassay (RIA) method using antiserum to cortisol-21-succinyl-BSA was devised to determine cortisol in fish plasma using the plasma of cod Gadus morhua. Steroid extracts were purified by thin layer chromatography before RIA. Saturated ammonium sulfate was used to separate protein-bound from free [3H]cortisol. The method is precise and accurate and validated by double isotope derivative assay (DIDA). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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