Thiazole orange: A new dye for reticulocyte analysis
| Autor: | Linda G. Lee, Chia-Huei Chen, Laura A. Chiu |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Reticulocytes
Chemical structure Biophysics Quantum yield Cell Count Pathology and Forensic Medicine Flow cytometry chemistry.chemical_compound Endocrinology Reticulocyte Fluorescence microscope medicine Humans Benzothiazoles Fluorescent Dyes Chromatography medicine.diagnostic_test DNA Cell Biology Hematology Flow Cytometry Fluorescence Thiazoles medicine.anatomical_structure chemistry Biochemistry Quinolines RNA Thioflavin Cytometry |
| Zdroj: | Cytometry. 7:508-517 |
| ISSN: | 1097-0320 0196-4763 |
| DOI: | 10.1002/cyto.990070603 |
| Popis: | The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitation, was used as a model. Several dyes were synthesized and evaluated by quantum yield determination, fluorescence microscopy, and flow cytometry. The best results were obtained with a dye we have named "thiazole orange"; analysis of several blood samples with thiazole orange gave a correlation coefficient of 0.97 as compared to a manual determination of reticulocyte percentage. |
| Databáze: | OpenAIRE |
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