Comparison of DNA Sequencing Analysis with Phenotyping Test for Identification of Yeasts Isolated from Foods

Autor:	Tomoaki Wauke, Satoshi Morozumi, Kazuyoshi Yano, Kenji Sadamasu, Takashi Chiba
Rok vydání:	2007
Předmět:	Genetics Sequence analysis Food Contamination Genes rRNA RNA Fungal Sequence Analysis DNA General Medicine Biology Polymerase Chain Reaction Yeast DNA sequencing Phenotype Species level Yeasts Food Microbiology Identification (biology) DNA Fungal
Zdroj:	Journal of the Food Hygienic Society of Japan (Shokuhin Eiseigaku Zasshi). 48:1-7
ISSN:	1882-1006 0015-6426
DOI:	10.3358/shokueishi.48.1
Popis:	The identification of 20 strains of yeasts isolated from foods by means of DNA sequence analysis with two kinds of universal primers for the rDNA region was examined, and the results were compared with those of the conventional phenotyping test using API 20C AUX. In the analysis of the 26S region, all 20 yeast strains tested were identified at the species level. In the ITS1 region, 16 strains were also classified at the species level. In addition, all results of DNA sequence analysis were consistent with those of the phenotyping test at the genus level. Furthermore, DNA sequence analysis was able to identify causative yeasts observed in two suspect foods, though phenotyping tests alone failed to identify them.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d386fd9ebf1f026128dad6409a4e49a0 https://doi.org/10.3358/shokueishi.48.1 Zobrazit plný text záznamu