Regulatory T lymphocytes and transforming growth factor beta in epithelial ovarian tumors-prognostic significance
| Autor: | Krzysztof Postawski, Barbara Wilczynska, Tomasz Rechberger, Aneta Adamiak, Izabela Winkler, Dorota Darmochwal-Kolarz, Agnieszka Bojarska-Junak, Jacek Tabarkiewicz, Marek Gogacz |
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| Jazyk: | angličtina |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Pathology medicine.medical_specialty Regulatory T lymphocyte medicine.medical_treatment Ovary Carcinoma Ovarian Epithelial T-Lymphocytes Regulatory Ovarian tumor Benign tumor of ovary Blood serum Ovarian cancer Transforming Growth Factor beta Obstetrics and Gynaecology medicine Neoplasm Humans Neoplasms Glandular and Epithelial Aged Ovarian Neoplasms business.industry Research Obstetrics and Gynecology Cancer FOXP3 Middle Aged medicine.disease Prognosis medicine.anatomical_structure Cytokine Oncology Immunology Female business |
| Zdroj: | Journal of Ovarian Research |
| ISSN: | 1757-2215 |
| DOI: | 10.1186/s13048-015-0164-0 |
| Popis: | Background Regulatory T lymphocytes (Treg) are characterized by the presence of CD4+ surface antigen. Today the transcription factor FOXP3 is considered to be the most specific marker of Treg cells. The aim of the study was to estimate the percentage of Treg in peripheral blood and the tissue of the epithelial ovarian tumor and blood serum TGF-beta concentrations and relationships between them. Moreover, the aim of the study was to answer the question whether the percentage of Treg lymphocytes affects the time of survival in patients with ovarian cancer. Methods The patients were divided into four groups, depending on the histopathological examination result: I – a group without any pathology within the ovaries (C; n = 20), II – a group with benign tumors (B; n = 25), III – with borderline tumors (BR; n = 11), IV – a group with cancer of the ovary (M; n = 24). The percentage of Treg lymphocytes in peripheral blood and the tissue was assessed using the flow cytometry method. TGF-beta cytokine concentration was estimated with the ELISA immunoenzymatic test. Statistical analysis of the results was conducted using the computer program Statistica 10.0PL (StatSoft, Inc). Results No significant differences were found in percentages of Treg lymphocytes in peripheral blood between individual groups of patients (p = 0.11). However, we observed marked differences in the tissue of malignant and non-malignant tumors between individual groups of patients (p = 0.003). The analysis with the post hoc test revealed significantly higher TGF-beta concentration in the group of women with malignant tumors. Moreover, no relationship was found between TGF-beta concentration and the percentage of Treg cells in peripheral blood and tumors of the ovary. No correlation was found between the percentage of Treg lymphocytes in peripheral blood (p = 0.4) and the tissue of ovarian tumors (p = 0.3) and the time of survival of patients with ovarian cancer. Conclusions The recruitment of Treg lymphocytes toward the tumor is one of the mechanisms of escape of neoplasm from the response of the immune system. The percentage of Treg lymphocytes in peripheral blood and the neoplastic tissue does not influence the time of survival of patients with ovarian cancer. |
| Databáze: | OpenAIRE |
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