EDTA-dependent pseudothrombocytopenia in patients with hepatosplenic schistosomiasis mansoni: a clinical management alert
| Autor: | Silvana Junia Roriz, Thiago A. Pereira, Júlia Fonseca de Morais Caporali, Debora Vaz de Melo Trindade, Patrícia Donado Vaz de Melo, José Roberto Lambertucci, Guilherme Vaz de Melo Trindade |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
030231 tropical medicine Schistosomiasis 030204 cardiovascular system & hematology Gastroenterology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Sodium citrate medicine Humans Platelet In patient Edetic Acid Platelet Count business.industry Hepatosplenic schistosomiasis Public Health Environmental and Occupational Health Anticoagulants General Medicine medicine.disease Thrombocytopenia Schistosomiasis mansoni Agglutination (biology) Infectious Diseases Blood smear chemistry Pseudothrombocytopenia Parasitology business |
| Zdroj: | Transactions of The Royal Society of Tropical Medicine and Hygiene. 115:1168-1173 |
| ISSN: | 1878-3503 0035-9203 |
| DOI: | 10.1093/trstmh/trab034 |
| Popis: | Background Hepatosplenic schistosomiasis mansoni (HS) is associated with thrombocytopenia. Accurate platelet counts are required for identification and management of HS patients. EDTA-dependent pseudothrombocytopenia (EDTA-PTCP) is an in vitro phenomenon of anticoagulant-activated platelet agglutination resulting in low platelet counts by automated methods. The prevalence of EDTA-PCTP in schistosomiasis is unknown and only one case has been described. Our aims were to determine the prevalence of EDTA-PTCP in HS and evaluate alternative methods to overcome this analytical error. Methods Blood samples from 56 HS patients and 56 healthy volunteers were collected, and platelet counts were obtained using standard microscopy and automated (electric impedance) methods. Automated platelet counts and the presence of platelet clumps in blood smears were evaluated in samples collected in EDTA or sodium citrate tubes 20 and 180 min after blood collection. Results EDTA-PTCP was more frequent in HS patients than healthy volunteers (8.92% vs 0.00%, p Conclusions Automated platelet counts in blood samples from HS patients should be performed right after blood collection in EDTA tubes and verified by manual counts in blood smears. |
| Databáze: | OpenAIRE |
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