Human chorionic gonadotropin and its free β-subunit stimulate trophoblast invasion independent of LH/hCG receptor

Autor: William S.B. Yeung, Philip C.N. Chiu, Laura Hautala, Tuula Salo, Cheuk-Lun Lee, Ulf-Håkan Stenman, Hannu Koistinen
Rok vydání: 2013
Předmět:
Male
Gene Expression
Chorionic Gonadotropin
Biochemistry
Human chorionic gonadotropin
0302 clinical medicine
Endocrinology
Cell Movement
Chorionic Gonadotropin
beta Subunit
Human
RNA
Small Interfering
Receptor
reproductive and urinary physiology
0303 health sciences
biology
Chemistry
Receptors
LH
Trophoblasts
medicine.anatomical_structure
Matrix Metalloproteinase 9
Enzyme Induction
Gene Knockdown Techniques
030220 oncology & carcinogenesis
embryonic structures
Matrix Metalloproteinase 2
Female
hormones
hormone substitutes
and hormone antagonists
ATP synthase alpha/beta subunits
Protein Binding
endocrine system
medicine.medical_specialty
03 medical and health sciences
Paracrine signalling
Cell Line
Tumor
Internal medicine
medicine
Humans
Embryo Implantation
Autocrine signalling
Molecular Biology
Cell Proliferation
030304 developmental biology
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinase-1
urogenital system
Choriocarcinoma
Trophoblast
medicine.disease
Urokinase-Type Plasminogen Activator
Cell culture
biology.protein
Zdroj: Molecular and Cellular Endocrinology. 375:43-52
ISSN: 0303-7207
Popis: Both paracrine and autocrine factors are involved in the regulation of trophoblast invasion. One of these factors is human chorionic gonadotropin (hCG), which stimulates trophoblast invasion. The stimulatory activity has especially been ascribed to a hyperglycosylated form of hCG (hCG-h) that is expressed in early pregnancy. We compared the stimulatory activities of different forms of hCG and its free β-subunit (hCGβ) on trophoblast invasion. hCG, hCG-h, hCGβ, and its hyperglycosylated form (hCGβ-h) stimulated the invasion of JEG-3 choriocarcinoma cells. The stimulatory effect of hCGβ was also confirmed with primary human trophoblasts. Down-regulation of the LH/hCG receptor by RNA-interference did not significantly reduce the effect of hCGβ and hCG on cell invasion. Increased invasion was associated with increased levels of MMP-2, MMP-9 and activity of uPA. Our findings suggest that hCG, hCGβ and their hyperglycosylated forms stimulate the invasion of trophoblast cells independent of the classical LH/hCG-receptor.
Databáze: OpenAIRE
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