Identification of a specifically expressed modified form of novel PSP-94 protein in the secretion of benign prostatic hyperplasia
| Autor: | Tian-Yong Fan, Kexin Xu, Davy T. Lee, James J. W. Xuan, Ming-Tat Ling, Xianghong Wang, Yong-Chuan Wong, Sai Wah Tsao, Franky L. Chan |
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| Rok vydání: | 2003 |
| Předmět: |
Gene isoform
PCA3 Male medicine.medical_specialty Prostatic Secretory Proteins Clinical Biochemistry Prostatic Hyperplasia Biology Biochemistry Analytical Chemistry Internal medicine medicine Humans Protein Isoforms Secretion Electrophoresis Gel Two-Dimensional Gene Aged Gel electrophoresis Reverse Transcriptase Polymerase Chain Reaction Alternative splicing Hyperplasia Middle Aged medicine.disease Molecular biology Neoplasm Proteins Alternative Splicing Endocrinology Case-Control Studies Protein Processing Post-Translational |
| Zdroj: | Electrophoresis. 24(7-8) |
| ISSN: | 0173-0835 |
| Popis: | Using two-dimensional gel electrophoresis, we investigated the profile of prostatic secretory proteins in human expressed prostatic secretion (EPS) from benign prostate hyperplasia (BPH) patients and compared the patterns with normal controls. We identified three specifically expressed proteins, including prostate secretory protein 61 (PSP61), in EPS from benign BPH patients but absent in normal controls. In addition, we found that PSP61 was a modified isoform of the well-documented PSP-94, which had a perfect matching (100% homology) to the first 61 amino acids of the PSP-94 protein but with a deleted C-terminus. This shortened PSP61 was not due to alternative splicing of the PSP-94 gene at transcription level. Our results provide first evidence on the possibility of using PSP61 as a specific biological marker for diagnosis of BPH. |
| Databáze: | OpenAIRE |
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