Survival of pseudorabies virus on swabs maintained under standard field sample shipping conditions
| Autor: | M. B. McCaw, Maria T. Correa, Jinsheng Xu |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
0301 basic medicine
Veterinary medicine 040301 veterinary sciences Alginates Swine 030106 microbiology Palatine Tonsil Pseudorabies Biology Virus Specimen Handling 0403 veterinary science 03 medical and health sciences Glucuronic Acid Chlorocebus aethiops Animals Vero Cells Gossypium General Veterinary Inoculation Hexuronic Acids 04 agricultural and veterinary sciences biology.organism_classification Virology Herpesvirus 1 Suid Titer |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 7(1) |
| ISSN: | 1040-6387 |
| Popis: | Pseudorabies virus survival was compared using three different types of applicator swabs in Eagle's minimum essential medium held under shipping conditions (packed with frozen gel packs) for up to 96 hours. Virus titer decay rates for dacron-tipped applicators were not statistically different from those of controls. Titer decay rates were statistically different from controls for cotton- and calcium alginate-tipped applicators. With the lowest input virus titer, virus was detectable up to 96, 72, or 24 hours after inoculation for dacron-, cotton-, and calcium alginate-tipped applicators, respectively. Dacron-tipped applicators were chosen to evaluate pseudorabies virus survival on tonsil swabs collected from experimentally challenged or contact control pigs to simulate field sampling and shipping conditions. Virus was still detectable in 20 of 24 swab samples after 72 hours in cell culture medium under shipping conditions. |
| Databáze: | OpenAIRE |
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