Molecular Characteristics, Virulence Gene and Wall Teichoic Acid Glycosyltransferase Profiles of Staphylococcus aureus: A Multicenter Study in China
Autor: | Mengyuan Xiong, Junying Zhou, Lijun Wang, Xuehan Li, Jun Lin, Yirong Li, Yunbao Pan, Xiao Xiao, Jin Zhao, Weihua Wang, Tao Huang, Zhijun Zhao |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Microbiology (medical)
Staphylococcus aureus lcsh:QR1-502 Virulence Biology medicine.disease_cause Microbiology glycosyltransferase lcsh:Microbiology molecular characterization 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance Staphylococcus epidermidis medicine Gene 030304 developmental biology Original Research 0303 health sciences Teichoic acid virulence gene 030306 microbiology Glycosyltransferase Gene biology.organism_classification chemistry wall choice acid Mobile genetic elements |
Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 11 (2020) |
ISSN: | 1664-302X |
Popis: | Staphylococcus aureus (S. aureus) constantly evolves under host and environment pressures. The monitoring network is essential in assessing the epidemiology of S. aureus infections. A total of 555 S. aureus isolates were collected from five hospitals in three different geographical regions of China for the investigation of molecular characteristics, antibiotic resistance, virulence gene, and wall teichoic acid (WTA) glycosyltransferase gene profiles. 233 (42.0%) isolates were identified as MRSA, and 323 (58.2%) were defined as multidrug-resistant (MDR) isolates. MRSA prevalence showed no significant difference among the three regions. In contrast, the MDR prevalence was significantly higher in central China than that in northern China (63.5% vs. 50.8%, P < 0.05). Thirty-eight sequence types (STs) belonging to 17 clone complexes (CCs) and 126 distinct spa-types were identified. The most prevalent clone was ST59-t437 (9.7%, 54/555), followed by ST22-t309 (7.6%, 42/555) and ST5-t2460 (7.2%, 40/555). Most ST59-t437 and ST5-t2460 were MRSA isolates, whereas most ST22-t309 was MSSA isolates. The predominant clones varied in different geographical areas. The distribution of the pvl, etb, tsst, clfb, sdrC, sdrD, hlg, fnbA, and hla genes showed significant differences among different regions. We found five WTA glycosyltransferase gene profiles, with tarP-/tarS+/tarM-/tagN- being the most common combination. Remarkably, the tarP gene was identified in more CCs than just CC5 and CC398. All of 16 tarP-positive isolates also contained the tarS. Moreover, tarS was present in almost all S. aureus isolates except 10 ST630 isolates. The tagN gene was only detected in 10 of 12 ST630 S. aureus isolates without tarS. The tarM gene was absent in CC5 and CC398. In brief, there were regional differences among molecular characteristics, antibiotic resistance, and virulence gene profiles. The tarS-negative ST630 lineage carried the tagN, which was never found before, indicating that it may be capable of expressing GroP-α-GalNAc WTA and exchanging mobile genetic elements with coagulase-negative staphylococci (CoNS). |
Databáze: | OpenAIRE |
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