Unexpected translation initiation within the coding region of eukaryotic genes expressed in Escherichia coli
| Autor: | Hideki Ishihara, Michael Leineweber, Gerald Preibisch, Dominique Tripier |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Genetics
Messenger RNA Base Sequence Transcription Genetic Molecular Sequence Data General Medicine EIF4A1 Biology Blotting Northern Eukaryotic translation initiation factor 4 gamma Recombinant Proteins Ribosomal binding site Eukaryotic translation Genes Eukaryotic initiation factor Escherichia coli Initiation factor Humans Nucleic Acid Conformation RNA Cloning Molecular Peptide Chain Initiation Translational Gene |
| Zdroj: | Gene. 72(1-2) |
| ISSN: | 0378-1119 |
| Popis: | When expressing several eukaryotic genes in Escherichia coli, we observed N-terminally truncated proteins which were attributed to translation initiation at downstream AUG codons. These AUG codons are located between 4 and 20 nucleotides 3' from sequences resembling bacterial SD elements. Although the presence of such downstream SD sequences is not sufficient for downstream initiation to occur, in two cases their removal abolishes synthesis of the truncated proteins. In one construct, a potential hairpin-loop structure is likely to inhibit translation initiation at the correct site and favor downstream initiation. |
| Databáze: | OpenAIRE |
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