Salivary leptin induces increased expression of growth factors in oral keratinocytes
| Autor: | W. Rascher, M. Rauh, Dötsch J, Kratzsch J, Hans-Georg Topf, Michael Gröschl |
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| Rok vydání: | 2005 |
| Předmět: |
Keratinocytes
Leptin medicine.medical_specialty Saliva Fibroblast Growth Factor 7 medicine.medical_treatment Biology chemistry.chemical_compound Endocrinology Epidermal growth factor Internal medicine medicine Humans Molecular Biology Cells Cultured Cell Proliferation Leptin receptor Dose-Response Relationship Drug Epidermal Growth Factor Cell growth Growth factor Mouth Mucosa Fibroblast Growth Factors medicine.anatomical_structure Gene Expression Regulation chemistry Keratinocyte growth factor Keratinocyte Signal Transduction |
| Zdroj: | Journal of Molecular Endocrinology. 34:353-366 |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1677/jme.1.01658 |
| Popis: | We investigated the influence of salivary leptin, purified by affinity chromatography, on the proliferation of human oral keratinocytes. Furthermore we determined the time- and dose-dependency of the incubation with salivary leptin on the production of epidermal growth factor (EGF) and keratinocyte growth factor (KGF), which are growth factors relevant to keratinocyte proliferation. The analysis was performed both intra- and extracellularly. The relationship between the three cytokines in cell proliferation was studied by successive blocking with specific antibodies. The incubation of oral keratinocytes with recombinant and native leptin led to a significantly increased, dose-dependent cell proliferation (PPPb (the long form of the leptin receptor) expressed by the keratinocytes is functional. It is conceivable that the leptin-induced proliferation in keratinocytes is mediated by this signalling pathway. This is the first study to show a physiological role of salivary leptin as a growth factor for keratinocyte proliferation in the oral cavity. We could demonstrate its influence on the production of other growth factors essential for this proliferative effect. Based on the findings of our study we assume an important role for salivary leptin in wound healing within the vulnerable oral cavity. |
| Databáze: | OpenAIRE |
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