Malondialdehyde precursors in gamma-irradiated DNA, deoxynucleotides and deoxynucleosides
| Autor: | William A. Haseltine, William D. Henner, Mike F. Janicek |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Free Radicals
Nitrogen Stereochemistry Thiobarbituric acid Deoxyribonucleotides Nitrous Oxide Deoxyribonucleosides Biology chemistry.chemical_compound Malondialdehyde Genetics Moiety Nucleotide chemistry.chemical_classification Air DNA Thiobarbiturates Malonates Thymine Oxygen Biochemistry chemistry Deoxyribose Gamma Rays Thymidine |
| Zdroj: | Nucleic Acids Research. 13:9011-9029 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/13.24.9011 |
| Popis: | Gamma-irradiation of DNA, deoxynucleosides, or deoxynucleotides produces material that reacts with thiobarbituric acid to form a chromophore with maximum absorbance at 532 nm. This material is not malondialdehyde. We have identified a new radiation product (thymin-1'-yl)-propenal as the TBA-reactive product of gamma-irradiation of thymidine. Thymine-propenal has been described by other investigators as a product of bleomycin-treatment of DNA. Irradiation of thymidine nucleotides produces phosphorylated precursors to thymine-propenal. Studies of the requirements for formation of TBA-reactivity indicate a mechanism involving reaction of a free radical with the deoxyribose moiety and molecular oxygen. On the basis of these results it is proposed that gamma-irradiation produces TBA-reactive material in DNA by the same reaction sequence in which bleomycin catalyzes the formation of base-propenals in DNA. Bleomycin and gamma-irradiation differ in the extent to which the sequence proceeds to completion with release of free base-propenals. |
| Databáze: | OpenAIRE |
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