Immunomodulatory activity and chemical characterisation of sangre de drago (dragon's blood) from Croton lechleri
| Autor: | Ester Risco, Elida Alvarez, José Iglesias, Roser Vila, Salvador Cañigueral, Felipe Ghia |
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| Rok vydání: | 2003 |
| Předmět: |
DPPH
Croton lechleri Complement Pathway Alternative Pharmaceutical Science Biology Pharmacology Carrageenan Analytical Chemistry chemistry.chemical_compound Mice Adjuvants Immunologic Phagocytosis Picrates Drug Discovery medicine Leukocytes Animals Edema Humans Plant Oils Complement Pathway Classical chemistry.chemical_classification Reactive oxygen species Monocyte Organic Chemistry Taspine Anti-Inflammatory Agents Non-Steroidal Biphenyl Compounds Biological activity Free Radical Scavengers biology.organism_classification Flow Cytometry Croton Rats Biphenyl compound medicine.anatomical_structure Complementary and alternative medicine chemistry Biochemistry Molecular Medicine Phytotherapy |
| Zdroj: | Planta medica. 69(9) |
| ISSN: | 0032-0943 |
| Popis: | The immunomodulatory activity of the latex from Croton lechleri (sangre de drago) was determined by in vitro assays. Classical (CP) and alternative (AP) complement pathways activities were determined in human serum. Intracellular generation of reactive oxygen species (ROS) by human polymorphonuclear leukocytes (PMNs) and monocytes, and phagocytosis of opsonised fluorescent microspheres were measured by flow cytometry. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Activity on proliferation of murine lymphocytes was also investigated. In addition, anti-inflammatory activity was assayed in vivo by carrageenan-induced rat paw oedema test. Some of the activities were compared with those of the isolated alkaloid taspine. Sangre de drago from Croton lechleri showed immunomodulatory activity. It exhibited a potent inhibitory activity on CP and AP of complement system and inhibited the proliferation of activated T-cells. The latex showed free radical scavenging capacity. Depending on the concentration, it showed antioxidant or prooxidant properties, and stimulated or inhibited the phagocytosis. Moreover, the latex has strong anti-inflammatory activity when administered i. p. Taspine cannot be considered the main responsible for these activities, and other constituents, probably proanthocyanidins, should be also involved. |
| Databáze: | OpenAIRE |
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